Preclinical research of the experimental preparation “Ferosel T”
The results of preclinical studies of the new ferrodextran preparation “Ferosel T”, which contains ferrum and selenium, are presented. It has been established that at oral administration of ferrocellus T in a prophylactic dose of 2 ml/kg b.w. and the therapeutic dose of 4 ml/kg for 3 days in a row in the stomach of laboratory rats did not show toxic effects. No toxic effect of T ferrocellus is established at introducing it into the stomach in doses in 3 and 10 times higher than the therapeutic ones. Under conditions of subcutaneous administration of the drug, the death of white rats was not observed, only short-term inhibition of laboratory animals, which was prescribed the drug in a dose of 10 ml/kg b.w. It was established that in the preventive and optimal therapeutic doses the drug did not affect the detoxification function of the liver. In rats, which ferrocellus T was administered at maximum therapeutic and possibly toxic doses, the duration of hexenal sleep was for 32 and 35% higher relative to control values. The investigation of the emotional and behavioral reactions of laboratory animals after administration of ferrocellus T for 21 days in therapeutic and maximum therapeutic doses did not show a significant effect on the nervous system. In terms of hyperemia and swelling of the skin and the thickness of the skin, ferrocellus T in prophylactic and therapeutic doses upon application to rabbit skin did not cause local irritation. Separate injection of the drug “Ferosel T” by sub-planar way to guinea pigs in 0.1 ml. did not cause swollen reactions of the paws. As a result of the conducted research, no allergenic properties of the drug “Ferosel T” were found. In laboratory rats, which were introduced ferrosel T in the prophylactic dose of probable changes in the weight of the heart, liver, spleen and kidneys is not established. In rats, which were administered ferrocellus T in an optimal therapeutic dose, in comparison with control weights of the spleen and liver, respectively, was in 10.3 and 6.4% higher. When introducing ferrocellus T at the maximum therapeutic dose, the mass of the spleen and liver was in 14.0 and 15.0% higher, respectively. The results of the studies indicate that the drug “Ferosel T” is safe when used for the prevention and treatment of animals.
Danchuk, V.V., Prystupa, T.I., & Polishchuk, O.I. (2013). Dynamika kholesterolu lipoproteidiv u krovi porosiat-sysuniv pry vvedenni preparativ Fe. Veterynarna biotekhnolohiia, 22, 117–122. Rezhym dostupu: http://nbuv.gov.ua/UJRN/ vbtb_2013_22_24 (in Ukrainian).
Gutyj, B., Grymak, Y., Hunchak, V., Mysak, A., Nazaruk, N., Brezvyn, O., Hariv, I., Shcherbatyy, A., Semeniv, B., Bushueva, I., Parchenko, V., & Kaplaushenko, A. (2018). Preclinical searches of the preparation Thireomagnile. Ukrainian Journal of Ecology, 8(1), 688–695. doi: 10.15421/2018_267.
Gutyj, B., Khariv, I., Binkevych, V., Binkevych, O., Levkivska, N., Levkivskyj, D., & Vavrysevich, Y. (2017). Research on acute and chronic toxity of the experimental drug Аmprolinsyl. Regul. Mech. Biosyst., 8(1), 41–45. doi: 10.15421/021708.
Gutyj, B., Martyshchuk, T., Bushueva, I., Semeniv, B., Parchenko, V., Kaplaushenko, A., Magrelo, N., Hirkovyy, A., Musiy, L., & Murska, S. (2017). Morphological and biochemical indicators of blood of rats poisoned by carbon tetrachloride and subject to action of liposomal preparation. Regulatory Mechanisms in Biosystems, 8(2), 304–309. doi: 10.15421/021748.
Gutyj, B., Paska, M., Levkivska, N., Pelenyo, R., Nazaruk, N., & Guta, Z. (2016). Study of acute and chronic toxicity of ‘injectable mevesel’ investigational drug. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(2), 174–180. doi: 10.15421/201649.
Hunchak, R.V., Sedilo, H.M., Kystsiv, V.O., Gutyj, B.V., & Hunchak, V.M. (2018). Total liquid maintenance and correlation of their classes in the sow’s colostrum and milk at different levels of aquacart of Iodine in their rations. Ukrainian Journal of Ecology, 8(1), 644–648. doi: 10.15421/2017_261.
Khariv, M., Gutyj, B., Butsyak, V., & Khariv, I. (2016). Hematological indices of rat organisms under conditions of oxidative stress and liposomal preparation action. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6 (1), 276–289. doi: 10.15421/201615.
Khariv, M., Gutyj, B., Ohorodnyk, N., Vishchur, O., Khariv, I., Solovodzinska, I., Mudrak, D., Grymak, C., & Bodnar, P. (2017). Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug. Ukrainian Journal of Ecology, 7(4), 536–541. doi: 10.15421/2017_157.
Kryshtalska, M., Hunchak, V., & Gutyj, B. (2017). Influence of the drug «Trifuzol» on the functional state of the liver in chickens for eymeriozic invasion. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 19(77), 76–79. doi: 10.15421/nvlvet7718.
Lee, J.C., & Downing, S.E. (1979). Effects of anemia and growth retardation on respiratory activity in the piglet. Biol Neonate, 36(5-6), 255-263. doi: 10.1159/000241237.
Martyshuk, T.V., Gutyj, B.V., & Vishchur, O.I. (2016). Level of lipid peroxidation products in the blood of rats under the influence of oxidative stress and under the action of liposomal preparation of «Butaselmevit», Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(2), 22–27. doi: 10.15421/201631.
Ohorodnyk, N.Z. (2013). Vplyv kompleksnoho liposomalnoho preparatu na hematolohichnyi profil krovi vidluchenykh porosiat. Svynarstvo, 62, 135–140. Rezhym dostupu: http://nbuv.gov.ua/UJRN/svun_2013_62_31 (in Ukrainian).
Pu, Y., Guo, B., Liu, D., Xiong, H., Wang, Y., & Du, H. (2015). Iron Supplementation Attenuates the Inflammatory Status of Anemic Piglets by Regulating Hepcidin. Biol Trace Elem Res., 167(1), 28–35. doi: 10.1007/s12011-015-0295-6.
Rozin, D.G. (1964). Modern evaluation of toxicity chlorproductive carbohydrates of fatty raw yder gexanal test with white mice. Pharmacology and toxicology. 5, 613–614.
Rytych, J.L., Elmore, M.R., Burton, M.D., Conrad, M.S., Donovan, S.M., Dilger, R.N., & Johnson, R.W. (2012). Early life iron deficiency impairs spatial cognition in neonatal piglets. J Nutr., 142(11), 2050–2056. doi: 10.3945/jn.112.165522.
Sobolev, O., Gutyj, B., Petryshak, R., Pivtorak, J., Kovalskyi, Y., Naumyuk, A., Petryshak, O., Semchuk, I., Mateusz, V., Shcherbatyy, A., & Semeniv, B. (2018). Biological role of selenium in the organism of animals and humans. Ukrainian Journal of Ecology, 8(1), 654–665. doi: 10.15421/2017_263.
Starzyński, R.R., Laarakkers, C.M., Tjalsma, H., Swinkels, D.W., Pieszka, M., Styś, A., Mickiewicz, M., & Lipiński, P. (2013). Iron supplementation in suckling piglets: how to correct iron deficiency anemia without affecting plasma hepcidin levels. PLoS One, 8(5), e64022. doi: 10.1371/journal.pone.0064022.
Todoriuk, V.B., Gutyj, B.V., Khomyk, R.I., & Vasiv, R.O. (2016). Influence of ferrovet 7.5% and ferosel T on the concentration of mineral substances in the blood serum of piglets suffering from Iron deficit anemia. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 3(71), 139–143. doi: 10.15421/nvlvet7131.
Zimmermann, W. (1995). Effects of different anemia prevention forms on the blood parameters of the suckling piglet. Dtsch Tierarztl Wochenschr, 102(1), 32–38. https://www.ncbi.nlm.nih.gov/pubmed/7781536.
This work is licensed under a Creative Commons Attribution 4.0 International License.