The effect of the drug “Bendamine” on the clinical and morphological parameters of dogs in heart failure

Keywords: pharmacology, Bendamine, heart failure, cardiomyopathy, dogs

Abstract

The study aimed to investigate the effect of the drug “Bendamine” on dogs' clinical and morphological parameters in heart failure. Two groups of dogs were formed for research: control (healthy) and experimental (sick). The 30-day experiment included ten dogs from the control group, ten dogs of the observed (unhealthy) spaniel breed, and 10–12 years old dogs. The treatment regimen for sick dogs: furosemide was administered orally on an empty stomach at 2 mg/kg every 12 hours; enalapril was administered orally at 0.5 mg/kg every 12 hours; Bendamine was administered orally on an empty stomach at 20 mg/kg every 24 hours. It is recommended to divide the dose into two doses. In the study of hematopoiesis, we found that erythrocytes in dogs with heart failure increased by 18.2 %, while hemoglobin levels decreased by 1.8 %. Before treatment, red blood cell counts were found to reduce the mean hemoglobin in the erythrocyte and the mean erythrocyte volume. In the experimental group of dogs treated with the “Bendamine”, normalization of morphological blood parameters was found. The number of erythrocytes was 5.8 ± 0.06 T/L, and the hemoglobin level was 143.5 ± 4.7 g/L. The use of the drug “Bendamine” contributed to the gradual restoration of the functional state of the hematopoietic system in dogs with heart failure. This is also indicated by the red blood cell index of the dogs after the course of treatment. The therapy application in the experimental group significantly reduced the frequency and severity of shortness of breath and increased endurance during exercise. There was also a decrease in the frequency and duration of cough attacks. After treatment, signs, and intensity of ascites decreased in dogs of the experimental group. It has been suggested that this is due to an increase in myocardial contractile function due to the positive ionotropic action of Bendamine. The owners also recorded an improvement in appetite in animals after 30 days of therapy. Even in the dogs of the experimental group, after treatment, physical activity and emotional reactions improved. They were less depressed and more inclined to communicate with the owners. Thus, the use of the cardiac drug “Bendamine” in dogs with heart failure positively affected the restoration of hematopoiesis and the normalization of morphological parameters of the blood.

References

Borgarelli, M., & Buchanan, J. W. (2012). Historical review, epide-miology, and natural history of degenerative mitral valve disease. Journal of Veterinary Cardiology, 14(1), 93–101. DOI: 10.1016/j.jvc.2012.01.011.
Borgarelli, M., Tarducci, A., Zanatta, R., & Haggstrom, J. (2007). Decreased systolic function and inadequate hypertrophy in large and small breed dogs with chronic mitral valve insufficiency. Journal of Veterinary Internal Medicine, 21(1), 61–67. DOI: 10.1892/0891-6640(2007)21[61:dsfaih]2.0.co;2.
Borgarelli, M., Zini, E., D'Agnolo, G., Tarducci, A., Santilli, R. A., Chiavegato, D., Tursi, M., Prunotto, M., & Häggström, J. (2004). Comparison of primary mitral valve disease in German Shepherd dogs and in small breeds. Journal of Veterinary Car-diology, 6(2), 27–34. DOI: 10.1016/S1760-2734(06)70055-8.
Chetboul, V., & Tissier, R. (2012). Echocardiographic assessment of canine degenerative mitral valve disease. Journal of Veteri-nary Cardiology, 14(1), 127–148. DOI: 10.1016/j.jvc.2011.11.005.
Cornell, C. C., Kittleson, M. D., Torre, P. D., Haggstrom, J., Lom-bard, C. W., Pedersen, H. D., Vollmar, A., & Wey, A. (2004). Allometric scaling of M-mode cardiac measurements in normal adult dogs. Journal of Veterinary Internal Medicine, 18(3), 311–321. DOI: 10.1892/0891-6640(2004)18<311:asomcm>2.0.co;2.
Fox, P. R. (2012). Pathology of myxomatous mitral valve disease in the dog. Journal of Veterinary Cardiology, 14(1), 103–126. DOI: 10.1016/j.jvc.2012.02.001.
Hollmer, M., Willesen, J. L., Tolver, A., & Koch, J. (2017). Left atrial volume and function in dogs with naturally occurring myxomatous mitral valve disease. Journal of Veterinary Cardi-ology, 19(1), 24–34. DOI: 10.1016/j.jvc.2016.08.006.
Keene, B. W., Atkins, C. A., Bonagura, J. D., Fox, P. R., Hägg-ström, J., Fuentes, V. L., Oyama, M. A., Rush, J. E., Stepien, R., & Uechi, M. (2019). ACVIM consensus guidelines for the diagnosis and treatment of myxomatous mitral valve disease in dogs. Journal of Veterinary Internal Medicine, 33(3), 1127–1140. DOI: 10.1111/jvim.15488.
Larouche-Lebel, E., Loughran, K. A., & Oyama, M. A. (2019). Echocardiographic indices and severity of mitral regurgitation in dogs with preclinical degenerative mitral valve disease. Journal of Veterinary Internal Medicine, 33(2), 489 –498. DOI: 10.1111/jvim.15461.
Lavryshyn, Y. Y., Varkholyak, I. S., Martyschuk, T. V., Guta, Z. А., Ivankiv, L. B., Paladischuk, О. R., Murska, S. D., Gutyj, B. V., & Gufriy, D. F. (2016). The biological significance of the antioxidant defense system of animals body. Scientific Messen-ger LNUVMBT named after S.Z. Gzhytskyj, 18, 2(66), 100–111. DOI: 10.15421/nvlvet6622.
Lopez-Alvarez, J., Elliott, J., Pfeiffer, D., Chang, Y.-M., Mattin, M., Moonarmart, W., Hezzell, M.J., & Boswood, A. (2015). Clini-cal Severity Score System in Dogs with Degenerative Mitral Valve Disease. Journal of Veterinary Internal Medicine, 29(2), 575–581. DOI: 10.1111/jvim.12544.
Martyshuk, T. V. (2016). The influence of oxidative stress on the state of the antioxidant defense system in the organism of rats. Regulatory Mechanisms in Biosystems, 7(1), 8–12. DOI: 10.15421/021602.
Martyshuk, T. V., & Hutyi, B. V. (2021). Imunofiziolohichnyi stan ta antyoksydantnyi potentsial orhanizmu porosiat za umov oksydatsiinoho stresu ta dii koryhuiuchykh chynnykiv: mon-ohrafiia. Lviv: SPOLOM (in Ukrainian).
Martyshuk, T. V., Gutyj, B. V., & Vishchur, O. I. (2016). Level of lipid peroxidation products in the blood of rats under the influ-ence of oxidative stress and under the action of liposomal prepa-ration of “Butaselmevit”. Biological Bulletin of Bogdan Khmelnitsky Melitopol State Pedagogical University, 6(2), 22–27. DOI: 10.15421/201631.
Martyshuk, T. V., Gutyj, B. V., & Vishchur, O. I. (2018). Indica-tors of functional and antioxidant liver status of rats under oxida-tive stress conditions and on the action of the liposomal drug “Butaselmevit”. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(89), 100–107. DOI: 10.32718/nvlvet8919.
Martyshuk, T. V., Gutyj, B. V., Vishchur, O. I., & Todoriuk, V. B. (2019). Biochemical indices of piglets blood under the action of feed additive “Butaselmevit-plus”. Ukrainian Journal of Veteri-nary and Agricultural Sciences, 2(2), 27–30. DOI: 10.32718/ujvas2-2.06.
Martyshuk, T., & Gutyj, B. (2019). Influence of feed additive “Bu-taselmevit-Plus” on antioxidant status of rats in conditions of ox-idative stress. Scientific Messenger of LNU of Veterinary Medi-cine and Biotechnologies. Series: Agricultural Sciences, 21(90), 76-81. DOI: 10.32718/nvlvet-a9013.
Menciotti, G., Borgarelli, M., Aherne, M., Wesselowski, S., Haggstrom, J., Ljungvall, I., Lahmers, S. M., & Abbott, J. A. (2017). Mitral valve morphology assessed by three-dimensional transthoracic echocardiography in healthy dogs and dogs with myxomatous mitral valve disease. Journal of Veterinary Cardi-ology, 19(2), 113–123. DOI: 10.1016/j.jvc.2017.01.002.
Merveille, A.-C., Bolen, G., Krafft, E., Roels, E., Gomart, S., Etienne, A.-L., Clercx, C., & Entee, K. Mc. (2015). Pulmonary Vein-to-Pulmonary Artery Ratio is an Echocardiographic Index of Congestive Heart Failure in Dogs with Degenerative Mitral Valve Disease. Journal of Veterinary Internal Medicine, 29(6), 1502–1509. DOI: 10.1111/jvim.13634.
Morgan, K. R. S., Monteith, G., Raheb, S., Colpitts, M., & Fonfara, S. (2020). Echocardiographic parameters for the assessment of congestive heart failure in dogs with myxomatous mitral valve disease and moderate to severe mitral regurgitation. The Veteri-nary Journal, 263, 1–25. DOI: 10.1016/j.tvjl.2020.105518.
Nazaruk, N. V., Gutyj, B. V., Gufrij, D. F., Leskiv, Kh. Ya., Ivash-kiv, R. M., & Martyshuk, T. V. (2021). The effect of methyphen and Vitamix se on the level of products of bull lipid peroxide ox-idation under nitrate-cadmium load. Colloquium-journal, 7(94), 16–18. DOI: 10.24412/2520-6990-2021-794-16-18
Oldach, M. S., Ueda, Y., Ontiveros, E. S., Fousse, S. L., Harris, S. P., & Stern, J. A. (2019). Cardiac Effects of a Single Dose of Pimobendan in Cats With Hypertrophic Cardiomyopathy; A Randomized, Placebo-Controlled, Crossover Study. Front Vet Sci., 6, 15. DOI: 10.3389/fvets.2019.00015.
Ramírez, V. L., Berrío, A., & Arias, M. P. (2016). Correlation between the clinical stage, echocardiographic findings and sys-temic blood pressure in dogs with Degenerative Disease of the Mitral Valve. CES Veterinary medicine and Zootechnics, 11(2), 61–72. DOI: 10.21615/cesmvz.11.2.5.
Reynolds, C. A., Brown, D. C., Rush, J. E., Fox, P. R., Nguyenba, T. P., Lehmkuhl, L. B., Gordon, S. G., Kellihan, H. B., Stepien, R. L., Lefbom, B. K., Meier, C. K., & Oyama, M. A. (2012). Prediction of first onset of congestive heart failure in dogs with degenerative mitral valve disease: The PREDICT cohort study. Journal of Veterinary Cardiology, 14(1), 193–202. DOI: 10.1016/j.jvc.2012.01.008.
Tilley, P. L., Francis, W. K., Smith, J., Oyama, M.A. (2008). Manu-al of canine and feline cardiology. Copyright by Saunders, an imprint of Elsevier Inc, 105–106 (Fourth edition).
Tjostheim, S. S., Kellihan, H. B., Grint, K. A., & Stepien, R. L. (2019). Effect of sildenafil and pimobendan on intracardiac heartworm infections in four dogs. J Vet Cardiol., 23, 96–103. DOI: 10.1016/j.jvc.2019.02.001.
Trofimiak, R., & Slivinska, L. (2021). Diagnostic value of echocar-diographic indices of left atrial and ventricular morphology in dogs with myxomatous mitral valve disease (MMVD). Ukraini-an Journal of Veterinary and Agricultural Sciences, 4(1), 16–23. DOI: 10.32718/ujvas4-1.04.
Trofimjak, R., & Slivinska, L. (2016). Diagnosis of chronic heart failure dogs – existing methods and future prospects. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 18(71), 130–133. DOI: 10.15421/nvlvet7129.
Undhad, V. V., Fefar, D. T., Jivani, B. M., Gupta, H., Ghodasara, D. J., Joshi, B. P. & Prajapati, K. S. (2012). Cardiac troponin: an emerging cardiac biomarker in animal health. Vet. World, 5, 508–511. DOI: 10.5455/vetworld.2012.508-511.
Varcholyak, I. S., & Gutyi, B. V. (2019). Determination of the chronic toxicity of preparation “Bendamin” on laboratory ani-mals. Theoretical and Applied Veterinary Medicine, 7(2), 63‒68. DOI: 10.32819/2019.71011.
Varkholiak, I. S., & Gutyj, B. V. (2018). Determination of acute toxicity of “Bendamin” drug in laboratory animals. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(92), 209–212. DOI: 10.32718/nvlvet9243.
Varkholiak, I. S., & Gutyj, B. V. (2019). The degree of cumulation of the “Bendamin” drug in the body of white rats. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 21(94), 82–85. DOI: 10.32718/nvlvet9415.
Varkholiak, I. S., Gutyj, B. V., Leskiv, Kh. Ya., Kushnir, V. I., Hariv, I. I., Martyshuk, T. V., & Guta, Z. A. (2021). The effect of bendamine on antioxidant protection of rats' myocardium in doxorubicin intoxication. Colloquium-journal, 7(94), 18–21. DOI: 10.24412/2520-6990-2021-794-18-21
Varkholiak, I. S., Gutyj, B. V., Leskiv, Kh. Ya., Kushnir, V. I., Hariv, I. I., Martyshuk, T. V., & Guta, Z. A. (2021). The effect of bendamine on antioxidant protection of rats' myocardium in doxorubicin intoxication. Colloquium-journal, 7(94), 18‒21. DOI: 10.24412/2520-6990-2021-794-18-21.
Varkholiak, I., Gutyj, B., & Leskiv, Kh. (2020). Influence of “Ben-damin” on the indices of antioxidant protection in rat’s blood by an experimental doxorubicin-induced cardiomyopathy. Scientific Light, 35, 41–44.
Varkholiak, I., Gutyj, B., Kushnir, V., Nazaruk, N., Lisnyak, O., & Yurynets, T. (2020). The effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense sys-tem of blood in rats in experimental doxorubicin-induced cardi-omyopathy. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(100), 36–40. DOI: 10.32718/nvlvet10007.
Vlizlo, V. V., Fedoruk, R. S., & Raty`ch, I. B. (2012). Laboratorni metody` doslidzhen` u biologiyi, tvary`nny`cztvi ta vetery`narnij medy`cy`ni: dovidny`k. L`viv: Spolom (in Ukrainian).
Yata, M., Kooistra, H. S., & Beijerink, N. J. (2019). Cardiorenal and endocrine effects of synthetic canine BNP1-32 in dogs with compensated congestive heart failure caused by myxomatous mi-tral valve disease. J Vet Intern Med., 33(2), 462–470. DOI: 10.1111/jvim.15416.
Zhulikova, O. A. (2016). Monitoring rasprostranenija serdechno-sosudistyh zabolevanij sredi koshek i sobak v g. Blagovesh-hensk amurskoj oblasti. Dal'nevostochnyj agrarnyj vestnik, 2(38), 49–56. https://cyberleninka.ru/article/n/monitoring-rasprostraneniya-serdechno-sosudistyh-zabolevaniy-sredi-koshek-i-sobak-v-g-blagoveschensk-amurskoy-oblasti (in Rus-sian).
Published
2021-12-31
How to Cite
Varkholiak, I. S., Gutyj, B. V., Gufriy, D. F., Sachuk, R. M., Mylostyvyi, R. V., Radzykhovskyi, M. L., Sedilo, H. M., & Izhboldina, O. O. (2021). The effect of the drug “Bendamine” on the clinical and morphological parameters of dogs in heart failure. Ukrainian Journal of Veterinary and Agricultural Sciences, 4(3), 76-83. https://doi.org/10.32718/ujvas4-3.13

Most read articles by the same author(s)