The activity of T- and B-cell links of specific protection of chicken-broilers under the influence of synbiotic preparation “Biomagn” and “Diolide” disinfectant

Keywords: broiler chickens, pro- and prebiotics, chlorine dioxide, immunity, nonspecific resistance

Abstract

One of the most actual problems of modern poultry farming is to increase the preservation of chickens and ensure high intensity of their growth at all stages of rearing. The development of alternative methods of prevention and treatment of infectious diseases of poultry involves the use of preparations of natural or organic origin, which have antagonistic properties against infectious agents and are able to balance the immune response. A prominent place in the system of disease prevention and increasing the resistance of animals and poultry is the development of modern probiotic and bactericidal preparations. In view of this, we studied the influence of the synbiotic preparations “Biomagn” in combination with the disinfectant “Diolide” on the state of the T- and B-cell links of the specific immunity of broiler chickens during the period of their rearing. The preparation “Biomagn” is based on representatives of the normal commensal microflora - non-pathogenic lactic acid bacteria with antibacterial and immunomodulatory properties and additional components: magnesium chloride, chitosan, xylanase, protease, cellulase, milk thistle meal, acidity regulator, betaine and emulsifier. The specified remedy is used to ensure optimal level of metabolism, increase immune function, growth, safety of animals and poultry. The results of the research showed that the use of the synbiotic preparation “Biomagn” in combination with the disinfectant “Diolide” had a positive influence on the activity of T- and B-cell links of specific defense of broiler chickens organism during the period of their rearing. This is evidenced by an increase in the amount of T-lymphocytes (total, active and theophylline-resistant) and B-lymphocytes in the blood and an increase in their functional activity due to the redistribution of the receptor apparatus of immunocompetent cells. In particular, a decrease in the number of functionally inactive T- and B-lymphocytes in the blood and an increase in the number of cells with low and medium degree of avidity. Therefore, the use of a set of studied immunotropic preparations is a promising direction in increasing the resistance and productivity of poultry. This allows in industrial conditions of maintenance, without loss of productivity, to respond to technological factors that can lead to immunodeficiency, disease and death of poultry.

References

Akpolat, M., Kanter, M., & Uzal, M. C. (2009). Protective effects of curcumin against gamma radiation-induced ileal mucosal damage. Archives of Toxicology, 83(6), 609–617. https://doi.org/10.1007/s00204-008-0352-4

Avdosieva, I. K. (2008). Aktualni pytannia veterynarnoho zakhystu v ptakhivnytstvi. Efektyvne ptakhivnytstvo, 4, 44–48 (in Ukrainian).

Barratt, M. D., & Rodford, R. A. (2001). The computational prediction of toxicity. Current Opinion in Chemical Biology, 5(4), 383–388. https://doi.org/10.1016/s1367-5931(00)00218-0

Brezvyn, O. M., Guta, Z. A., Gutyj, B. V., Fijalovych, L. M., Karpovskyi, V. I., Shnaider, V. L., Farionik, T. V., Dankovych, R. S., Lisovska, T. O., Bushuieva, I. V., Parchenko, V. V., Magrelo, N. V., Slobodjuk, N. M., Demus, N. V., & Leskiv, Kh. Ya. (2021). The influence of HamekoTox on the morphological and biochemical indices of the blood of laying hens in spontaneous fumonisin toxicosis. Ukrainian Journal of Ecology, 11 (2), 249–253. https://www.ujecology.com/articles/the-influence-of-hamekotox-on-the-morphological-and-biochemical-indices-of-laying-hens-blood-in-spontaneous-fumonisin-to.pdf

Catlin, N. R., Willson, C. J., Creasy, D. M., Rao, D. B., Kissling, G. E., McIntyre, B. S., & Wyde, M. (2018). Evaluation of the respiratory tract toxicity of orthophthalaldehyde, a proposed alternative for the chemical disinfectant glutaraldehyde. Inhalation Toxicology, 29(9), 414–427. https://doi.org/10.1080/08958378.2017.1390015

Ducle, H., Hong, H. A., Barbosa, T. M., Henriques, A. O., & Cutting, S. M. (2004). Characterization of Bacillus probiotics available for human use. Applied and Environmental Microbiology, 70(4), 2161–2171. https://doi.org/10.1128/AEM.70.4.2161-2171.2004

Frei, R., Akdis, M., & O'Mahony, L. (2015). Prebiotics, probiotics, synbiotics, and the immune system: experimental data and clinical evidence. Current Opinion in Gastroenterology, 31(2), 153–158. https://doi.org/10.1097/MOG.0000000000000151

Hrabovskyi, S. S., & Hrabovska, O. S. (2015). Vplyv imunomoduliatoriv pryrodnoho pokhodzhennia na pokaznyky klitynnoho imunitetu krovi kurchat-broileriv v umovakh stresu. Visnyk Dnipropetrovskoho universytetu. Biolohiia, medytsyna, 6(1), 36–39 (in Ukrainian). https://doi.org/10.15421/021507

Kalinichenko, S. V. (2013). Suchasnyi stan rozrobky ta zastosuvannia probiotychnykh, prebiotychnykh ta synbiotychnykh preparativ. Annaly Mechnykovskoho instytutu, 3, 5–12 (in Ukrainian). http://nbuv.gov.ua/UJRN/ami_2013_3_3

Kaminska, M. V., Nechay, H. I., Tsepko, N. I., & Kolisnyk, H. V. (2008). Influence carotenecontaining yeast biomass and β-carotene on the microfloras composition and immune status of rats. The Animal Biology, 10(1–2), 261–265.

Kim, J. S., Hosseindoust, A., Lee, S. H., Choi, Y. H., Kim, M. J., Lee, J. H., Kwon, I. K., & Chae, B. J. (2017). Bacteriophage cocktail and multi-strain probiotics in the feed for weanling pigs: effects on intestine morphology and targeted intestinal coliforms and clostridium. Animal, 11(1), 45–53. https://doi.org/10.1017/s1751731116001166

Klaenhammer, T. R., Kleerebezem, M., Kopp, M. V., & Rescigno, M. (2012). The impact of probiotics and prebiotics on the immune system. Nature Reviews Immunology, 12(10), 728–734. https://doi.org/10.1038/nri3312

Kogan, G., & Kocher, A. (2007). Role of yeast cell wall polysaccharides in pig nutrition and health protection. Livestock Science, 109(1-3), 161–165. https://doi.org/10.1016/j.livsci.2007.01.134

Kovalenko V. L. (2008). Metody vyznachennia imunotoksychnoi dii dezinfikuiuchykh zasobiv. Veterynarna biotekhnolohiia, 13(1), 266–272 (in Ukrainian).

Kovalenko, V. L., Kvachov, V. H., & Yashchenko, M. F. (2005). Imunolohichna bezpeka zastosuvannia dezinfikuiuchykh preparativ. Naukovo-tekhnichnyi biuleten Instytutu biolohii tvaryn ta DNDKI vetpreparativ i kormovykh dobavok, 6(3), 139–143 (in Ukrainian).

Kukkonen, K., Savilahti, E., Haahtela, T., Juntunen-Backman, K., Korpela, R., Poussa, T., Tuure, T., & Kuitunen, M. (2007). Probiotics and prebiotic galacto-oligosaccharides in the prevention of allergic diseases: a randomized, double-blind, placebo-controlled trial. Journal of Allergy and Clinical Immunology, 119(1), 192–198. https://doi.org/10.1016/j.jaci.2006.09.009

Kurtiak, B. M., & Romanovych M. M. (2015). Zastosuvannia probiotykiv u ptakhivnytstvi – osnova epizootychnoho blahopoluchchia ptakhohospodarstv. Naukovyi visnyk LNUVMB imeni S. Z. Gzhytskoho, 17(2(62), 100–102 (in Ukrainian). http://nbuv.gov.ua/UJRN/nvlnu_2015_17_2_23

Kuzmenko, O. V. (2011). Daily rhythm of immune system indexes rats and radio sensitivity. Experimental and Сlinical Medicine, 1(50), 132–141.

Lesyk, Y. V., Dychok-Niedzielska, A. Z., Boiko, О. V., Honchar, О. F., Bashchenko, М. І., Kovalchuk, І. І., & Gutyj, B. V. (2022). Hematological and biochemical parameters and resistance of the organism of mother rabbits receiving sulfur compounds. Regulatory Mechanisms in Biosystems, 13(1), 60–66. https://doi.org/10.15421/022208

Ma, J.-W., Huang, B.-S., Hsu, C.-W., Peng, C.-W., Cheng, M.-L., Kao, J.-Y., Way, T.-D., Yin, H.-C., & Wang, S.-S. (2017). Efficacy and Safety Evaluation of a Chlorine Dioxide Solution. International Journal of Environmental Research and Public Health, 14(3), 329. https://doi.org/10.3390%2Fijerph14030329

Manzanares, W., Lemieux, M., Langlois, P. L., & Wischmeyer, P. E. (2016). Probiotic and synbiotic therapy in critical illness: a systematic review and meta-analysis. Critical Care, 19, 262. https://doi.org/10.1186/s13054-016-1434-y

Marvar, P. J., Vinh, A., Thabet, S. Lob, H. E., Geem, D., Ressler, K. J., & Harrison, D. G. (2012). T lymphocytes and vascular inflammation contribute to stress-dependent hypertension. Biological Psychiatry, 71(9), 774–782. https://doi.org/10.1016/j.biopsych.2012.01.017

Mokienko, A. V. Lozickij, V. P., Petrenko, N. F., Gridina, T. L., & Grigorasheva, I. N. (2007). Gigienicheskaja ocenka virulicidnogo dejstvija dioksida hlora po otnosheniju k virusu ptich'ego grippa. Zahalna patolohiia ta patolohichna fiziolohiia, 2(5), 100–104 (in Ukrainian).

Mokiienko, A. V., & Petrenko, N. F. (2008). Hihiienichna otsinka virulitsydnoi dii dioksydu khloru po vidnoshenniu do priorytetnykh enterovirusiv pytnoi vody ta stichnykh vod. Dosiahnennia biolohii ta medytsyny, 2(12), 52–57 (in Ukrainian). http://dspace.nbuv.gov.ua/handle/123456789/47374

Mokrozub, V. V., Lazarenko, L. M., & Babenko, L. P. (2012). Antybakterialni y imunomoduliuvalni vlastyvosti shtamiv lakto- ta bifidobakterii za eksperymentalnoi stafilokokovoi infektsii. Biotekhnolohiia, 5(2), 98–104 (in Ukrainian). http://nbuv.gov.ua/UJRN/biot_2012_5_2_11

Mokrozub, V. V., Lazarenko, L. M., Babenko, L. P. et al. (2012). Effect of probiotic strains of lacto- and bifidobacteria on the activity of macrophages and other parameters of immunity in cases of staphylococcosis. Mikrobiolohichnyi zhurnal, 74(6), 90–98. https://pubmed.ncbi.nlm.nih.gov/23293832

Mudrak, D. I., & Vishchur, O. I. (2011). Kilkist T- i V-limfotsytiv ta yikh funktsionalna aktyvnist u krovi husei za riznoho rivnia vitaminiv E i S u ratsioni. Biolohiia tvaryn, 13(1-2), 427–430 (in Ukrainian). http://aminbiol.com.ua/2011pdf/67.pdf

Orishchuk, O. S., Tsap, S. V., & Mykytiuk, V. V. (2014). Produktyvnist ta yakisni pokaznyky yaiets kurei-nesuchok za zghodovuvannia kormovoi dobavky VAMZhK. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu, 7(26), 97–101 (in Ukrainian). http://nbuv.gov.ua/UJRN/Vsna_tvar_2014_7_24

Romanovich, M., Vishchur, O., Kurtyak, B., & Smolyaninov, K. (2017). Immunological reactivity and lipid peroxidation in broiler under the influence of BPS-44 drug and yeast Saccharomyces cerеvisiae. Aktualne problem w patologii drobiu–stare I nowe wyzwania istotne w produkcji drobiarskiej. Wroclaw, 164–168.

Romanyuk, S. I., & Komisarenko, S. V. (2012). Immunity: what do force its to work? Visnyk of National Academy of Sciences, 1, 49–54.

Sanekata, T., Fukuda, T., Miura, T., Morino, H., Lee, C., Maeda, K., Araki, K., Otake, T., Kawahata, T., & Shibata, T. (2010). Evaluation of the antiviral activity of chlorine dioxide and sodium hypochlorite against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus. Biocontrol Science, 15(2), 45–49. https://doi.org/10.4265/bio.15.45

Sarao, L. K., & Arora, M. (2017). Probiotics, prebiotics, and microencapsulation: A review. Critical Reviews in Food Science and Nutrition, 57(2), 344–371. https://doi.org/10.1080/10408398.2014.887055

Sazawal, S., Hiremath, G., Dhingra, U., Malik, P., Deb, S., & Black, R. E. (2006). Efficacy of probiotics in prevention of acute diarrhoea: a meta-analysis of masked, randomized, placebo-controlled trials. The Lancet Infectious Diseases, 6(6), 374–382. https://doi.org/10.1016/s1473-3099(06)70495-9

Sobolev, O. I., Gutyj, B. V., Sobolievа, S. V., Borshch, О. O., Nedashkivsky, V. M., Kachan, L. M., Karkach, P. M., Nedashkivska, N. V., Poroshinska, O. А., Stovbetska, L. S., Emelyanenko, A. A., Shmayun, S. S., & Guta, Z. A. (2020). Selenium in natural environment and food chains. A Review. Ukrainian Journal of Ecology, 10(4), 148–158. https://www.ujecology.com/articles/selenium-in-natural-environment-and-food-chains-a-review.pdf

Sobolev, O. I., Lisohurska, D. V., Pyvovar, P. V., Topolnytskyi, P. Р., Gutyj, B. V., Sobolievа, S. V., Borshch, О. O., Liskovich, V. А., Verkholiuk, M. M., Petryszak, O. Y., Kuliaba, O. V., Golodiuk, I. P., Naumjuk, O. S., Petryszak, R. A., & Dutka, H. I. (2021). Modeling the effect of different dose of selenium additives in compound feed on the efficiency of broiler chicken growth. Ukrainian Journal of Ecology, 11 (2), 292–299. https://www.ujecology.com/articles/modeling-the-effect-of-different-dose-of-selenium-additives-in-compound-feed-on-the-efficiency-of-broiler-chicken-growth.pdf

Sorlini, S., Gialdini, F., Biasibetti, M., & Collivignarelli, C. (2014). Influence of drinking water treatments on chlorine dioxide consumption and chlorite/chlorate formation. Water Research, 54, 44–52. https://doi.org/10.1016/j.watres.2014.01.038

Starovoitova, S. A., Babenko, L. P., Timoshok, N. A., Shynkarenko, L. N., Lazarenko, L. N., & Spivak, N. Y. (2012). Cholesterol-lowering activity of lactic acid bacteria probiotic strains in vivo. Mikrobiolohichnyi zhurnal, 74(3), 78–84. https://pubmed.ncbi.nlm.nih.gov/22830201

Stoianovskyi, V. H., & Kolomiiets, I. A. (2011). Probiotyky ta imunna systema shlunkovo-kyshkovoho traktu ptytsi. Suchasne ptakhivnytstvo, 4(101), 21–25 (in Ukrainian).

Tam, M. Gomez, S., Gonzalez-Gross, M., & Marcos, A. (2003). Possible roles of magnesium on the immune system. European Journal of Clinical Nutrition, 57, 1193–1197. https://doi.org/10.1038/sj.ejcn.1601689

Tizard, I. R. (2009). Veterinary immunology. An Introduction. 8th ed. Saunders Elsevier.

Tsynovyi, O. V. (2013). Vplyv dezinfektantiv na kliniko-biokhimichni ta hematolohichni pokaznyky kurchat-broileriv. Visnyk Poltavskoi derzhavnoi ahrarnoi akademii, 4, 96–99 (in Ukrainian). http://nbuv.gov.ua/UJRN/VPDAA_2013_4_27

Vlizlo, V. V. (2012). Laboratorni metody doslidzhennia v biolohii, tvarynnytstvi ta veterynarnii medytsyni: Dovidnyk. Lviv: Spolom (in Ukrainian).

Yu, C. H., Huang, T. C., Chung, C. C., Huang, H. H., & Chen, H. H. (2014). Application of highly purified electrolyzed chlorine dioxide for Tilapia fillet disinfection. The Scientific World Journal, 2014, Article ID 619038. https://doi.org/10.1155/2014/619038

Published
2022-04-27
How to Cite
Chechet, O. M., Kovalenko, V. L., Vishchur, O. I., Haidei, O. S., Liniichuk, N. V., Gutyj, B. V., & Krushelnytska, O. V. (2022). The activity of T- and B-cell links of specific protection of chicken-broilers under the influence of synbiotic preparation “Biomagn” and “Diolide” disinfectant. Ukrainian Journal of Veterinary and Agricultural Sciences, 5(1), 46-52. https://doi.org/10.32718/ujvas5-1.08

Most read articles by the same author(s)

1 2 3 > >>