Selection of the optimal composition of vegetable oil and chlorophyllipt oil components
Investigate the antimicrobial properties of various concentrations of vegetable essential oils in combination with an oil solution of chlorophyllipt as possible ingredients for ointments for wounds. Microbiological tests were performed according to standard methods using E. coli test cultures, S. aureus, Str. agalactiae and P. fluorescens. The results of studies of antibacterial activity of both individual ingredients and their combinations are presented, namely: 4 % essential oil of Siberian pine, 2 % essential oil of eucalyptus, 1.5 % essential oil of cloves, 1.5 % of essential oil of cedar, 2.0 % tea tree essential oil and 1.0 % chlorophyllipt oil solution. Bacteria, which are usually typical agents of wound infections, have been found to be quite sensitive to the drugs tested. High activity of essential oils and oily solution of chlorophyllipt with respect to E. coli and S. aureus was revealed. In particular, a 1.0 % oily solution of chlorophyllipt caused staphylococcal growth retardation zones whose diameters were 1.4 times larger than the antibiotic clindamycin. It was determined that representatives of gram-negative microflora were more sensitive to the investigated essential oils and chlorophyll. The optimal composition of the experimental drug called “Ointment for wounds” is offered. The results of preclinical testing showed a sufficiently high efficiency compared to traditional means. The results obtained with regard to antimicrobial activity indicate the prospect of using preparations based on the essential oil of Siberian pine, eucalyptus, carnation, cedar, tea tree and oil solution of chlorophyllipt for the treatment of skin diseases in animals. This data will help to develop new effective and safe veterinary treatments for wound care.
Bennouna, F., Lekbach, Y., Sadiki, M., El Abed, S., Koraichi, S.I., & Lachkar, M. (2018). Antimicrobial efficacy of three essential oils against decaying cedar wood isolates. Research Journal of Microbiology, 13(2), 119–126. doi: 10.3923/jm.2018.119.126.
Bushuieva, I. V. (2013). Marketynhovi doslidzhennia rozvytku rynku veterynarnykh preparativ ta oblasti veterynarnoi far-matsii. [Marketing research on the development of the veteri-nary market and the field of veterinary pharmacy]. Zaporizkyi medychnyi zhurnal, 78(3), 90–93. http://nbuv.gov.ua/UJRN/Zmzh_2013_3_25 (in Ukrainian).
Carrión-Prieto, P., Martín-Gil, J., Fernández-Coppel, I. A., Ruíz-Potosme, N. M., & Martin-Ramos, P. (2018). Physicochemical studies of Siberian pine (Pinus sibirica) derived chewing gum. Trends in Phytochemical Research, 2(2), 119–124.
European committee on antimicrobial susceptibility testing break-point tables for interpretation of MICs and zone diameters. Version 7.1, valid from 2017-03-10. Available online at: http://www.eucast.org.
European committee on antimicrobial susceptibility test-ing antimicrobial susceptibility testing. EUCAST disk diffu-sion method. Version 5.0. January 2015. Available online at: www.eucast.org.
Gerecke, U. (2005).Market Opportunities in Natural and Alterna-tive Animal Health Products. Richmond: T&F Informa UK Ltd.
Harkat-Madouri, L., Asma, B., Madani, K., Said, Z.B.O., Rigou, P., Grenier, D., & Boulekbache-Makhlouf, L. (2015). Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalyptus globulus from Algeria. Industrial Crops and Products, 78, 148–153. doi: 10.1016/j.indcrop.2015.10.015.
Hong, E. J., Na, K. J., Choi, I. G., Choi, K. C., & Jeung, E. B. (2004). Antibacterial and antifungal effects of essential oils from conifer-ous trees. Biological and Pharmaceutical Bulletin, 27(6), 863–866. doi: 10.1248/bpb.27.863.
Horiuk, Yu. V. (2018). Fagotherapy of cows mastitis as an alterna-tive to antibiotics in the system of obtaining environmentally safe milk. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(88), 42–47. doi: 10.32718/nvlvet8807.
Li, M., Zhu, L., Liu, B., Du, L., Jia, X., Han, L., & Jin, Y. (2016). Tea tree oil nanoemulsions for inhalation therapies of bacterial and fungal pneumonia. Colloids and Surfaces B: Biointerfaces, 141, 408–416. doi:10.1016/j.colsurfb.2016.02.017.
Okmen, G., Kardas, S., Bayrak, B., Arslan, A., & Cakar, H. (2016). The antibacterial activities of Crocus sativus against mastitis pathogens and its antioxidant activities. WJPPS, 5(3), 146–156.
Queiroga, M. C., Pinto Coelho, M., Arantes, S. M., Potes, M. E., & Martins, M. R. (2018). Antimicrobial activity of essential oils of Lamiaceae aromatic spices towards sheep mastitis-causing Staphylococcus aureus and Staphylococcus epidermidis. Jour-nal of Essential Oil Bearing Plants, 21(5), 1155–1165. doi: 10.1080/0972060X.2018.1491330.
Santa Packyanathan, J., & Prakasam, G. (2017). Antibacterial effect of clove oil against clinical strains of Escherichia coli. Journal of Pharmaceutical Sciences and Research, 9(7), 1203–1204. https://www.jpsr.pharmainfo.in/Documents/Volumes/vol9Issue07/jpsr09071734.pdf.
Santos, E. M. S., Almeida, A. C., Santos, H. O., Cangussu, A. R., Costa, K. S., Alves, J. N., Barbosa L. C. B., & Aguiar, R. W. S. (2019). Mechanism of Brassica oleracea performance in bo-vine infectious mastitis by bioinformatic analysis. Microbial pathogenesis, 129, 19–29. doi: 10.1016/j.micpath.2019.01.029.
Shaheen, M., Tantary, H. A., & Nabi, S. U. (2016). A treatise on bovine mastitis: disease and disease economics, etiological ba-sis, risk factors, impact on human health, therapeutic manage-ment, prevention and control strategy. Advances in Dairy Re-search, 10(1), 150. doi: 10.4172/2329-888X.1000150.
Shpatov, A. V., Popov, S.A., Salnikova, O.I., Kukina, T.P., Shmidt, E.N., & Um, B.H. (2017). Composition and bioactivity of lipophilic metabolites from needles and twigs of Korean and Siberian Pines (Pinus koraiensis Siebold & Zucc. and Pinus sibirica Du Tour). Chemistry & biodiversity, 14(2), e1600203. doi: 10.1002/cbdv.201600203.
Smith, A.C., Wood, C.L., McQuerry, K.J., & Bewley, J.M. (2014). Effect of a tea tree oil and organic acid footbath solution on digital dermatitis in dairy cows. Journal of dairy science, 97(4), 2498–2501. doi: 10.3168/jds.2013-6776.
Tamminen, L. M., Emanuelson, U., & Blanco-Penedo, I. (2018). Systematic review of phytotherapeutic treatments for different farm animals under European conditions. Frontiers in veteri-nary science, 5, 140. doi: 10.3389/fvets.2018.00140.
Vorobets, N. M., Kryvtsova, M. V., Rivis, O. Y., Spivak, M. Y., Yavorska, H. V., & Semenova, H. M. (2018). Antimicrobial activity of phytoextracts on opportunistic oral bacteria, yeast and bacteria from probiotics. Regulatory Mechanisms in Bio-systems, 9(3), 374–378. doi: 10.15421/021855.
Wolski, T., Najda, A., Kędzia, B., & Wolska-Gawron, K. (2017). Phytotherapy of dermatological diseases. Postępy Fitoterapii, 4, 298–308. doi: 10.25121/PF.2017.18.4.298.