Evaluation the effect of using thyme and oregano powder in comparison to the antibiotic and probiotic supplementation on growth, some immune responses and intestinal morphology of broiler chicks
To determine the effect of using thyme and oregano powder supplementation on performance, some immune response and intestinal morphology of broiler chicks a total of 200 one-day-old Cobb 500 male broiler chicks were divided into the four treatments consisting of five replicates of 10 chicks each. The treatments were as control groups with no herbal, antibiotic and probiotic supplementations. control groups + virginiamycine antibiotic, control groups + protexin probiotic, control groups + 2 % of each thyme and oregano powder supplementation and Control groups + 4 % of each thyme and oregano powder supplementation. The performance parameters such as feed intake, body weight gain and feed conversion ratio were measured during the study period. To determine immunity parameters on days 28, 0.5 mL of 10 % sheep red blood cells suspension was administrated to two chicks per each replicate through and at day 31, 3 mL blood samples were collected from left wing. On day 42, jejunum tissue samples were separated and fixed in (10 %) neutral buffer formalin and were analyzed by auto-processing apparatus, there after the slide sections were prepared by rotary microtome type (Manubeni, Erma-Tokyo, Japan) and the average of villi measurements was reported as a mean for each bird. The result of this study showed that the dietary inclusion of herbals and probiotic had no significant effects on FI and FCR of broiler chicks but higher body weight gain were seen with they received thyme+oregano supplementation. Inclusion of thyme+oregano and probiotic increased humeral immunity in broiler chicks compared to the control (P ≤ 0.05). Additionally heterophil, lymphocyte and their ratio were not influenced by experimental treatments. The villus length, villus width, and crypt depth were increased were the birds feed by oregano and thyme powders. In conclusion in current study some of beneficial acts toward using thyme and oregano powders on performance, some immune parameters and intestinal morphology on broiler chicks were demonstrated.
Amresh, G., Reddy, G., Rao, C. V., & Singh, P., (2007). Evaluation of anti-inflammatory activity of Cissampelospareira root in rats. Journal of Ethnopharmacology, 110(3), 526–531. doi: 10.1016/j.jep.2006.10.009.
AOAC. (2004). Official methods of analysis. 18th ed. Washington, DC: AOAC.
Attia, Y. A., Bakhashwain, A. A., & Bertu, N. K. (2017). Thyme oil (Thyme vulgaris L.) as a natural growth promoter for broiler chickens reared under hot climate. Italian Journal of Animal Science, 16(2), 275–282. doi: 10.1080/1828051X.2016.1245594.
Awad, W. A., Ghareeb, K., Abdel-Raheem, S., & Bohm, J., (2009). Effects of dietary inclusion of probiotic and synbi-otic on growth performance, organ weights, and intestinal histomor-phology of broiler chickens. Poultry Science, 88(1), 49–56. doi: 10.3382/ps.2008-00244.
Bai, K., Huang, Q., Zhang, J., He, J., Zhang, L., & Wang, T., (2017). Supplemental effects of probiotic Bacillus subtilis fmbJ on growth performance, antioxidant capacity, and meat quality of broiler chickens. Poultry Science, 96(1), 1–9. doi: 10.3382/ps/pew246.
Bilia, A. R., Guccione, C., Isacchi, B., Righeschi, C., Firenzuoli, F., & Bergonzi, M. C. (2014). Essential oils loaded in nanosystems: A developing strategy for a successful therapeutic approach. Evidences Based Complement Alternative Medicine, 2014, 651593. doi: 10.1155/2014/651593.
Dorman, H. J. D., & Deans, S. G., (2000). Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. Journal of Applied Microbiology, 88(2), 308–316. doi: 10.1046/j.1365-2672.2000.00969.x.
Gaggìa, F., Mattarelli, P., & Biavati, B., (2010). Probiotics and prebiotics in animal feeding for safe food production. International Journal of Food Microbiology, 141(1), S15–S28. doi: 10.1016/j.ijfoodmicro.2010.02.031.
Galal, A. A. A., El-Araby, I. E., Hassanin, O., & Omar, A. E. (2016). Positive impact of oregano essential oil on growth performance, humoral immune responses and chicken interferon alpha signalling pathway in broilers. Advance Animal Veterinary Science, 4(1), 57–65. doi: 10.14737/journal.aavs/2016/188.8.131.52.
Gartner, L. P., & Hiatt, J. L. (2001). Color Textbook of Histolo-gy.2nd ed. W. B. Saunders, Baltimore, MD.
Gross, W. B., & Siegel, H. S. (1983). Evaluation of the heterophil to lymphocyte ratio as a measure of stress in chickens. Avian Diseases, 27(4), 972–979. https://www.jstor.org/stable/pdf/ 1590198.pdf?seq=1.
Hashemipour, H., Kermanshahi, H., Golian, A., & Veldkamp, T. (2013). Effect of thymol and carvacrol feed supplementation on performance, antioxidant enzyme activities, fatty acid composition, digestive enzyme activities, and immune response in broiler chickens. Poultry Science, 92(8), 2059–2069. doi: 10.3382/ps.2012-02685.
Jahromi, M. F., Altaher, Y. W., Shokryazdan, P., Ebrahimi, R., Ebrahimi, M., Idrus, Z., Goh, Y. M., Tufarelli, V., & Liang, J. B. (2016). Dietary supplementation of a mixture of lactobacillus strains enhances performance of broiler chickens raised under heat stress conditions. International Journal of Biometeorology, 60(7), 1099–1110. doi: 10.1007/s00484-015-1103-x.
Kadam, A. S., Nikam, M. G., Patodkar, V. R., Muglikar, D. M., Lonkar, V. D., Yadav, G. B., Maini, S., Ravikanth, K., & Meshram, M. D. (2009). Influence of herbal early chick nutritional supplement on the growth performance,serumbiochemicals and immune response of broiler chicken. International Journal of Poultry Science, 8(4), 349–354. doi: 10.3923/ijps.2009.349.354.
Kotronia, M., Kavetsou, E., Loupassaki, S., Kikionis, S., Vouyiouka, S., & Detsi, A. (2017). Encapsulation of oregano (Origanum onites L.) essential oil in β-Cyclodextrin (β-CD): Synthesis and characterization of the inclusion complexes. Bioengineering, 4(3), 74. doi: 10.3390/bioengineering4030074.
Lee, K. W., Everts, H., & Kappert, H. J. (2004). Growth performance, intestinal viscosity, fat digestibility and plasma cholesterol in broiler chicks. International Journal of Poultry Science, 3(9), 613–618. doi: 10.3923/ijps.2004.613.618.
Lepage, K. T., Bloom, S. E., & Taylor, R. L. (1996). Antibody response to sheep red blood cells in a major histocompatibility (B) complex aneuploid line of chickens. Poultry Science, 75, 346–350.
Manach, F., Regerat, F., & Texier, O., (1996). Bioavailability, metabolism and physiological impact of 4-oxo-flavonoids. Neutral Research, 16(3), 517–544. doi: 10.1016/0271-5317(96)00032-2.
Mathlouthi, N., Bouzaienne, T., Oueslati, I., Recoquillay, F., Hamdi, M., Urdaci, M., & Bergaoui, R., (2012). Use of rosemary, oregano, and a commercial blend of essential oils in broiler chickens:In vitro antimicrobial activities and effects on growthperformance. Journal of Animal Science, 90(3), 813–823. doi: 10.2527/jas.2010-3646.
Paturi, G., Phillips, M. Jones, M., & Kailasapathy, K. (2007). Immune enhancing effects of Lactobacillus acidophilus, lafti l10 and Lactobacillus paracasei, lafti l26 in mice. International Journal of Food Microbiology, 115(1), 115–118. doi: 10.1016/j.ijfoodmicro.2006.10.007.
Pluske, J. R., Thompson, M. J., Atwood, C. S., Bird, P. H., Wil-liams, L. H., & Hartmenn, P. E. (1996). Maintenance of villus height and crypt depth, and enhancement of di-saccharide diges-tion and monosaccharide absorption, in piglets fed on cows’ whole milk after weaning. British Journal of Nutrition, 76(3), 409–422. doi: 10.1079/bjn19960046.
Pournazari, M., Qotbi, A.A.A., Seidavi, A.R., Corazzin, M., (2017). Prebiotics, probiotics and thyme (Thymus vulgaris) for broilers:performance, carcass traits and blood variables. Review Colombia CiencPecu, 30, 3–10. doi: 10.17533/udea.rccp. v30n1a01.
Ragga, N. M. Kornay, R. S., & Mohamad, F. (2016). Effects of thyme/or formic acid dietary supplementation on broiler performance and immunity. Agriculture and Agricultural Science Procedia, 10, 270–279. doi: 10.1016/j.aaspro.2016.09.064.
Ross. Ross 308 Broiler (2014). Nutrition specification manual. Midhlotian (UK): Aviagen Ltd.
Samadian, F., Saeed, Z., Armin, T., Mohammad, A. K. T., Zarbakht, A. P., & Pedram, G. (2013). Evaluation of some phytogenic feed additives in growingchicks diet. International Journal of Agriculture Research Review, 3, 35–43.
Seidavi, A., Dadashbeiki, M., Alimohammadi-Saraei, M., van den Hoven, R., Payan-Carreira, R., Laudadio, V., & Tufarelli, V. (2017). Effects of dietary inclusion level of a mixture of probiotic cultures and enzymes on broiler chickens immunity response. Environment Science Pollutant Research, 24, 4637–4644. doi: 10.1007/s11356-016-8206-8.
Sengul, T., Yurtseven, S., Cetin, M., Kocyigit, A., & Sogut, B. (2008). Effect of thyme T. vulgaris extracts on fattening performance, some blood parameters, oxidative stress and DNA damage in Japanese quail. Journal of Animal Feed Science, 17(4), 608–620. doi: 10.22358/jafs/66689/2008.
Simitzis, P. E., & Deligeorgis, S. G. (2011). The effects of natural antioxidants dietary supplementation on the properties of farm animal products. In Animal Feed: Types, Nutrition, Safety; Nova Science Publishers, Inc.: New York, NY, USA, 155–168.
Simitzis, P. E. (2017). Enrichment of animal diets with essential oils-a great perspective on improving animal performance and quality characteristics of the derived products. Medicines, 4(2), 35–45. doi: 10.3390/medicines4020035.
Stoica, R., Somoghi, R., & Ion, R. M., (2013). Preparation of chitosan– tripolyphosphatenanoparticles for the encapsulation of polyphenols extracted form rose hips. Journal of Nano Biostructre, 8(3), 955–963. http://chalcogen.ro/955_Stoica.pdf.
Tabidi, M. H., Mukhtar, A. M., & Mohammed. H. I. (2013). Effectsof probiotic and antibiotic on performance and growth attributes of broiler chicks. Global Journal of Medicinal Plant Research, 1, 136–142.
Tsirtsikos, P., Fegeros, K., Balaskas, C., Kominakis, A., & Mountzouris, K. C. (2012). Dietary probiotic inclusion level modulates intestinal mucin composition and mucosal mor-phology in broilers. Poultry Science, 91(8), 1860–1868. doi: 10.3382/ps.2011-02005.
Yadav, G. B., Kadam, A. S., Pachpande, A. M., Lambate, S. B., Lonkar, V. D., Maini, S., & Ravikanth, K. (2010). Post- hatch histomorphological studies of small intestinal development in chicks fed with herbal early chick nutritional supplement. International Journal of Poultry Science, 9(9), 851–855. doi: 10.3923/ijps.2010.851.855.
Yakhkeshi, S., Rahimi, S., & Gharib Naseri, K. (2011). The effects of comparison of herbal extracts, antibiotic, probiotic and organic acid on serum lipids, immune response, GIT microbial population, intestinal morphology and performance of broilers. Journal of Medicinal Plants Research, 10(37), 80–95. http://jmp.ir/article-1-236-fa.pdf.
Yang, C. M., Cao, G. T., Ferket, P. R., Liu, T. T., Zhou, L., Zhang, L., Xiao, Y., & Chen, A. G. (2012). Effects of probiotic, Clostridiumbutyricum, on growth performance, immune function, and cecal microflora in broiler chickens. Poultry Science, 91(9), 2121–2129. doi: 10.3382/ps.2011-02131.
Zhang, Z. F., Zhou, T. X., Ao, X., & Kim, I. H. (2012). Effects of β- glucan and Bacillus subtilis, on growth performance, blood profiles, relative organ weight and meat quality in broilers fed maize–soybean meal based diets. Livestock Science, 150(1-3), 419–424. doi: 10.1016/j.livsci.2012.10.003.