Some larval morphological characteristics of Camelostrongylus mentulatus and Nematodirus spathiger


Keywords: Morphological characteristics, L3 larvae, Camelostrongylus mentulatus, Nematodirus spathiger, antelopes.

Abstract

Monitoring of gastrointestinal nematode parasites in ruminants (domestic and wild) is often based on fecal examination techniques, looking for excreted eggs and larval forms using morphological keys. These, are more available in domestic ruminants, in which helminths are widely studied, than in wild ruminants.  This study tried to provide certain morphological elements that will help to recognize the L3 larvae of Camelostrongylus mentulatus and Nematodirus spathiger that could parasite either domestic or wild ruminants. For that, we resorted first to the culture of L3 larvae from fecal samples taken from African antelopes, and second by the microscopic characterization of each isolated larval morphological pattern previously identified by sequencing of its internal transcribed spacer (ITS-2) regions of the ribosomal DNA. The results of different microscopic captured images showed that Camelostrongylus mentulatus larva is 16 intestinal cells that measuring approximately 820 µm length, ‎≈ 25 µm wide, and ‎≈ 47 µm for its sheath tail extension and by this be closer to Teladorsagia circumcincta characteristics.  For Nematodirus spathiger, it possesses 8 gut cells and measuring about 1020 µm long, ‎≈ 25 µm wide, and‎ ≈ 143 µm for its sheath tail extension with specific tail appendages. Have done this, we were able to get some clarifications on the morphology of the studied larvae, and we believe thus that this study will contribute to the establishment of morphological identification keys especially for parasitic nematodes of wild ruminants.

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Published
2020-05-17
How to Cite
Saidi, A., Mimouni, R., Hamadi, F., & Oubrou, W. (2020). Some larval morphological characteristics of Camelostrongylus mentulatus and Nematodirus spathiger. Ukrainian Journal of Veterinary and Agricultural Sciences, 3(2), 7-11. https://doi.org/https://doi.org/10.32718/ujvas3-2.02