Dynamics of individual indicators of protein metabolism in the body of broiler chickens on the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”
The body of broiler chickens is characterized by a high metabolism, which promotes rapid growth and development, but their performance indicators are largely related to housing conditions, which are known to include a number of technological stressors. With the development of stress in the body of poultry changes the course of metabolic processes, which causes changes in all types of metabolism, including protein. The aim of the study was to determine the changes in individual indicators of protein metabolism in the body of broiler chickens against the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”. At 13th day of life, all clinically healthy poultry were exposed to combined stress – revaccination (intranasal Newcastle disease) plus cold stress (for 60 minutes by air conditioning and 5 °C in the vivarium). Material for research was selected for 3 days after the action of the stress factor (stage of anxiety), 13, 20 and 26 days after the action of the stress factor (the resistance stage). The material for the study was blood plasma, which determined the concentration of total protein, as well as the content of albumin, globulin fraction (α1, α2, β, γ). It was found that 3 days after the action of stress in chickens of Control group the development of adaptive reactions is manifested by the stability of the total protein content and redistribution of the fractional composition of plasma globulins in the form of increased albumin and α2-globulins. At different stages of development of the stage of resistance in birds of Control group, the content of total protein decreases by an average of 14.6 % due to the content of albumin by 6.9 % and α2-globulins – by 15.9 %, against the background of increasing α1- and γ-globulins by 23.1 and 33.5 % with the stabilization of individual studied indicators at the final stages of development of the resistance stage. The use in the diet of broilers feed “Reasil Humic Vet”, probiotic feed supplement “Laktin”, feed “Reasil Humic Health” under the influence of complex stress helps to increase the intensity of protein metabolism in poultry with the development of adaptation syndrome, as evidenced by the growth of total protein on average by 37.8 % (P < 0.05) and albumin – by 17.0 % (P < 0.05), which indicates an increase in the intensity of protein-synthesizing properties of the organism. In different periods of stress in the blood of broiler chickens Research groups there is an increase in the ratio of individual protein fractions, especially the content of γ-globulins - an average of 21.3 % (P < 0.05), which indicates an increase in the immune status of their body with a predominance of numerical values in the Research 1 group of poultry.
Kovalenko, A. M., Tkachev, A. V., Tkacheva, O. L., Gutyj, B. V., Prystupa, O. I., Kukhtyn, M. D., Dutka, V. R., Veres, Ye. M., Dashkovskyy, O. O., Senechyn, V. V., Riy, M. B., & Kotelevych, V. A. (2020). Analgesic effectiveness of new nanosilver drug. Ukrainian Journal of Ecology, 10(1), 300–306. doi: 10.15421/2020_47.
Nguyen, P. H., Greene, E., Donoghue, A., Huff, G., Clark, F. D., & Dridi, S. (2016). A new insight into cold stress in poultry production. Adv. Food. Technol. Nutr. Sci. Open. J., 2(1), 1–2. doi: 10.17140/AFTNSOJ-2-124.
Saki, А. А., Maleckey, М., Johari, R., Goudarzi, S. М., & Abdolmaleki, М. (2016). The effects of protein, amino acid, and dietary electrolyte balance on broiler chicken performance and blood parameters under heat stress. Acta Sci., Anim. Sci., 38(3), 111–118. doi: 10.4025/actascianimsci.v38i3.30747.
Sobolev, O. I., Gutyj, B. V., Sobolievа, S. V. … Leskiv, K. Y., & Guta, Z. A. (2019). Chemical composition, energy and biological value of broiler chicken meat caused by various doses of selenium. Ukrainian Journal of Ecology, 9(4), 622–627. https://www.ujecology.com/articles/chemical-composition -energy-and-biological-value-of-broiler-chicken-meat-caused-by-various-doses-of-selenium.pdf.
Sobolievа, S. V., Gutyj, B. V., & Sobolev, O. I. (2020). Сhanges in goslings’ blood pattern under the influence of different doses additives of selenium in forages. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Agricultural sciences, 22(92), 50–55. doi: 10.32718/nvlvet-a9209.
Stojanovskij, V. G., Kolomіiеts, І. A., Kamratska, О. І., & Kolontytskij, V. А. (2012). Fіzіologіchnij stan organіzmu kurchat-brojlerіv u kritichnі vіkovі perіodi pri zastosuvannі іmunokoregujuchih preparatіv na tlі vakcinacіi. Naukovyi visnyk LNUVMB imeni S. Z. Gzhytskoho, 14, 2(53), 236–239 (in Ukrainian).
Stojanovskij, V. G., Kolomіес, І. A., Garmata, L. S., & Kamracka, O. І. (2018). Zmіni morfofunkcіonalnogo stanu organіv endokrinnoі ta іmunnoі sistem perepelіv promislovogo viroshhuvannja za dіі stresu. Fіzіologіchnij zhurnal, 64(1), 25–33. doi: 10.15407/fz64.01.025 (in Ukrainian).
Tóthová, C., Sesztáková, E., Bielik, B., & Nagy, O. (2019). Chang-es of total protein and protein fractions in broiler chickens during the fattening period. Vet World, 12(4), 598–604. doi: 10.14202/vetworld.2019.598-604.
Tsiouris, V., Georgopoulou, I., Batzios, C., Pappaioannou, N., Ducatelle R., & Fortomaris P. (2015). The effect of cold stress on the pathogenesis of necrotic enteritis in broiler chicks. Avian Pathology, 44(6), 430–435, doi: 10.1080/03079457.2015.1083094.
Virden, W. S., & Kidd, M. T. (2009). Physiological stress in broilers: Ramifications on nutrient digestibility and responses. Journal of Applied Poultry Research, 18(2), 338–347. doi: 10.3382/japr.2007-00093.
Vlizlo, V. V., Fedoruk, R. S., & Ratіch, I. B. (2012). Laboratornі metodi doslіdzhen u bіologіі, tvarinnictvі ta veterinarnіj medicinі: dovіdnik (in Ukrainian).