Energy evaluation of cows using genetic markers


Keywords: Polymorphism in GH and PIT-1 genes, pure support energy, pure milk energy, energy and productivity index.

Abstract

The aim of the study was to research the polymorphism of allelic variants of somatotropin GH genes and pituitary-specific transcription factor PIT-1 and its influence on bioenergetic parameters of high-yielding of Holstein breed cows in intensive milk production technology (cold method of growing heifers, early insemination of heifers. high daily operating loads on the body, etc.). Genotypes of cows were determined by PCR. Blood DNA was examined in a genetic laboratory. Isolation of genomic DNA was performed using resin “Chelex-100”. The reaction was performed in an amplifier “Tertsyk”. For the energy assessment of first-born cows used indicators of net maintenance energy (MJ per day), net milk energy (MJ per day), total net energy consumption (MJ per day), the value of the energy index (the share of energy released from milk, %), productivity index (kilogram of 4 % milk per 1 MJ), net energy consumption per 1 MJ of milk (MJ), energy released with milk per 1 kg of metabolic mass (MJ). The best energy parameters in cows by GH gene – LL genotype, by pituitary-specific transcription factor PIT-1 – AB genotype, and among paired genotypes – LL/AB and LL/BB. In these animals, bioenergetic indicators for the first and second periods of lactation were higher than their peers of LV, BB and LV/BB genotypes by 2.0–15.2 %, with a reliable result for most traits. In particular, during the first lactation period, the advantage of cows of the LL/AB genotype over the LV/BB genotype in terms of bioenergetic indicators was 3.9–15.2 %, significantly less than in the second lactation period in the range of 1.8–7.4 %. In the genome of pituitary-specific transcription factor PIT-1, better bioenergetic parameters were found in cows of genotype AB than in cows of genotype BB. The difference in bioenergetic parameters is explained by the higher lactotropic function of the L and A alleles, and is the result of a greater complementary effect on the functioning of the alleles of the associated genes in the complex genotype. This was confirmed by the analysis of variance of one-factor complexes, which revealed a stronger influence of genotypes on bioenergetic parameters during the first period of lactation than the second. However, the strength of the influence of the paired genotype on the GH and PIT-1 genes was always high regardless of the lactation period and ranged from 8.9 to 20.9 % (P < 0.01–0.001). In the future, it is of interest to study the formation of the exterior and constitution of cows of different genetic influences under the influence of polymorphisms in the GH and PIT-1 genes.

References

Akyuz, B., Agaoglu, O. K., Akcay, A., & Agaoglu, A. R. (2015). Effects of DGAT1 and GH polymorphism on milk yield in Hol-stein cows reared in Turkey. Slovenian Veterinary Research, 52(4), 185–191. Available at: https://www.slovetres.si/index.php/SVR/article/view/147.

Burkat, V. P., Kopylov, K. V., & Kopylova, K. V. (2009). DNK-diagnostyka vely`koyi rogatoyi hudoby v systemi genomnoyi selekciyi [DNA diagnostics of cattle in the system of genomic selection] (metodychni rekomendaciyi). Kyyiv (in Ukrainian).

Chernenko, O. I. (2012). Produktyvnist ta rezultaty energetychnoyi ocinky koriv ukrayinskoyi chervonoyi molochnoyi porody zalezhno vid konstytucionalnyh osoblyvostej [Productivity and results of energy assessment of Ukrainian red dairy cows de-pending on constitutional features]. Naukovyj visnyk “Askaniya Nova”. “PYEL”, 5(2), 176–180 (in Ukrainian).

Chernenko, O. M., & Chernenko, O. I. (2018). Economic trait of cows with different duration of prenatal growth period. Theoret-ical and Applied Veterinary Medicine, 6(3), 23‒28. doi: 10.32819/2018.63005.

Denysyuk, O. V. (2009). Energetychna ocinka pervistok, otrymanyh vid batkiv riznogo ekogenezu [Energy assessment of first-borns received from parents of different ecogenesis]. Visnyk Instytutu tvarynnycztva centralnyh rajoniv UAAN, 6, 39–43 (in Ukraini-an).

Krupin, E. O., & Shakirov, Sh. K. (2019). Influence of CSN3, LGB, PRL, GH, TG5 genes alleles on dairy productivity and energy value of cow’s milk. Carpathian Journal of food science and technology, 11(4), 104–115. doi: 10.34302/2019.11.4.9.

Mylostyvyi, R., Chernenko, O., & Lisna, A. (2019). Prediction of comfort for dairy cows, depending on the state of the environ-ment and the type of barn. Monograph: Development of Modern Science: The Experience of European Countries and Prospects for Ukraine. doi: 10.30525/978-9934-571-78-7_53.

Petrenko, V. I., Barabash, V. I., & Docenko, L. V. (2005). Ener-getychna ocinka velykoyi rogatoyi hudoby [Energy assessment of cattle]. Rozvedennya i genetyka tvaryn, 39, 152–157 (in Ukrainian).

Shariflou, M. R., Moran, C., & Nicholas, F. W. (2000). Association of the Leu127 variant of the bovine growth hormone (bGH) gene with increased yield of milk, fat, and protein in Australian Holstein-Friesians. Australian Journal of Agricultural Research, 51(4), 515–522. doi: 10.1071/ar99102.

Trakovická, A., Vavrišínová, K., Gábor, M., Miluchová, M., Kasar-da, R., & Moravčíková, N. (2019). The impact of diacylglycerol O-acyltransferase 1 gene polymorphism on carcass traits in cat-tle. Journal of Central European Agriculture, 20(1), 12–18. doi: 10.5513/jcea01/20.1.2411.

VanRaden, P. M., & Sullivan, P. G. (2010). International genomic evaluation methods for dairy cattle. Genetics Selection Evolution, 42(1), 7. doi: 10.1186/1297-9686-42-7.

Zhao, Q., Davis, M. E., & Hines, H. C. (2004). Associations of polymorphisms in the Pit-1 gene with growth and carcass traits in Angus beef cattle12. Journal of Animal Science, 82(8), 2229–2233. doi: 10.2527/2004.8282229x.
Published
2020-08-19
How to Cite
Gubarenko, N. (2020). Energy evaluation of cows using genetic markers. Ukrainian Journal of Veterinary and Agricultural Sciences, 3(3), 3-7. https://doi.org/https://doi.org/10.32718/ujvas3-3.01