Light-scattering properties of microorganisms Desulfuromonas acetoxidans by influence of silver
The article deals with the concentration changes and relative content of bacterial cells of Desulfuromonas acetoxidans in the intervals of sizes 0.2–2.0 µm under the influence of nano silver particles. Correlation between these changes of light-scattering properties of bacterial cells and growth abilities of bacteria Desulfuromonas acetoxidans under inflence of silver nanoparticles and ions has been shown. The purpose of the work was to research the intensity of processes the change of indexes of the antioxidant system the cells of Desulfuromonas acetoxidans at influence of silver nanoparticles and silver nitrate. The influence of various concentrations of silver nanoparticles and silver nitrate on enzymatic activity of catalase and reduced glutathione synthesis by Desulfuromonas аcetoxidans cells under their cultivation with fumarate addition and with absence of sulphur has been determined. Specific catalase activity increased with enhancing of concentration and duration of bacterial cultivation under the addition of this salt. The highest specific catalase activity was determined on the second day of bacterial growth under the influence of all concentration range of investigated metal salt. The reduced glutathione content under silver nitrate and silver nanoparticles exposure varied depending on the cultivation time and metal concentration. The maximum reduced glutathione content has been observed. The result of catalase activity changes and glutathione content changes of sulfur-reducing D. acetoxidans bacteria cell-free extracts and has been investigated under the influence of different concentrations of Ag nanoparticles during four days of cultivation has been investigated.
Roden, E. E., & Lovley, D. R. (1993). Dissimilatory Fe(III) Reduc-tion by the Marine Microorganism Desulfuromonas acetoxidans. Аppl. Environ. Microbiol., 59, 734–742. URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC202183.
Bilyy, O., Vasyliv, O., & Hnatush, S. (2014). The Anode Biocata-lyst with Simultaneous Transition Metals Pollution Control. Technology and Application of Microbial Fuel Cells, Chin-Tsan Wang, Eds. Rijeka: InTech, InTech, 33–55. doi: 10.5772/58347.
Pfennig, N., & Biebl, H. (1976). Desulfuromonas acetoxidans gen. nov. and sp. nov., a new anaerobic, sulfur-reducing, acetate-oxidizing bacterium. Arch. Microbiol., 110(1), 3–12. doi: 10.1007/BF00416962.
Biebl, H., & Pfennig, N. (1977). Growth of sulfate-reducing bacteria with sulfur as electron acceptor. Arch. Microbiol., 112, 115–117. doi: 10.1007/BF00446664.
Bilyy, O. I., Getman, V. B., Bilyy, R. O., Kushnir, I. M., Kotsium-bas, I. Y. (2008). Rapid Detection of Bacterial Cells by Light Scattering Method. Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 686411. doi: 10.1117/12.762744.
Bilyy, O. I., Getman, V. B., Yaremyk, R. Y., Ferensovich, Y. P., Kotsiumbas, I. Y., Kushnir, I. M. (2011). A new device for reg-istration of bacterial cells. Proc. SPIE 8086, Advanced Micros-copy Techniques II, 80861K. doi: 10.1117/12.889604.
Goth, L. A. (1991). Simple method for determination of serum catalase activity and revision of reference range. Clin. Chim. Acta, 196(2-3), 143–151. doi: 10.1016/0009-8981(91)90067-m.
Owen, J. B. (2010). Butterfield DA Measurement of oxi-dized/reduced glutathione ratio. Methods Mol. Biol., 648, 269–277. doi: 10.1007/978-1-60761-756-3_18.