Protein-synthesizing function of the liver of cows at experimental fasciolosis


  • D. V. Frejuk Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Pekarska Str., 50, Lviv, 79010, Ukraine https://orcid.org/0000-0001-7076-3431
  • V. V. Stybel Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Pekarska Str., 50, Lviv, 79010, Ukraine https://orcid.org/0000-0002-0285-6182
Keywords: parasitology, ruminants, fasciolosis, liver, proteins, albumins, globulins

Abstract

Cattle fasciolosis is quite widespread in the world, including in Ukraine. Animal blood proteins, being in close functional connection with proteins of various tissues, reflect the changes that occur in the tissues and organs of the body during disorders of their metabolic processes caused by pathological factors. The aim of the study was to investigate the level of total protein and its fractions in the blood of cows in experimental fasciolosis. 12 cows of 4–5 years of age, black-spotted breed, were selected for experiment, out of which 2 groups were formed, six animals in each. Animals in the control group were clinically healthy. Animals of the experimental group were experimentally infected with adolescents. During the research, the rules of compulsory experiments were followed - selection and keeping of analogue animals in groups. The cows' diet was balanced in terms of nutrients and minerals. In the study of protein-sensitizing function of the liver of cows, it was found that during fasciolosis invasion, the content of total protein decreased by 13.8 %. These changes were due to a decrease in albumin and an increase in globulins. Thus, in fasciological invasion of cows, the level of albumin in the serum decreased by 24.4 %, and the level of globulins increased by 7.9 %. The value of the albumin / globulin ratio in the blood of infested cows with the causative agent of fasciolosis is quite indicative. It was found that the albumin-globulin ratio in the blood of cows of the experimental group was probably lower compared to the control group of animals. This value of the coefficient, of course, indicates the suppression of protein-synthesizing function of the liver in these periods of research. In the future it is planned to investigate the functional state of the liver of cows with experimental fasciolosis.

References

Aly, I. R., Hamad, R. S., Elshall, M., & Ahmed, M. (2020). Novel expression of Cathepsin B Fasciola gigantica sequence for detec-tion of human fascioliasis in Egypt. International journal of in-fectious diseases, 101(1), 251. doi: 10.1016/j.ijid.2020.11.091.

Ashrafi, K. (2015). The Status of Human and Animal Fascioliasis in Iran: A Narrative Review Article. Iran J Parasitol, 10(3): 306–328. URL: https://pubmed.ncbi.nlm.nih.gov/26622287.

Chernushkin, B. O., Vlizlo, V.V., Slivinska, L. G., Gutyj, B. V., Shcherbatyy, A. R., Maksymovych, I. A., Leno, M. I., Rusyn, V. I., Lychuk, M. H., Fedorovych, V. L., Lukashchuk, B. O., Zinko, H. O., Prystupa, O. I. (2020). Treatment strategies for sheep with acute yellow athrophy of the liver caused by the fasciolosis. Ukrainian Journal of Ecology, 10(2), 294–301. doi: 10.15421/2020_100.

Gutyj, B., Stybel, V., Hariv, I., Maksymovych, I., Buczek, K., Staniec, M., Milczak, A., Bushueva, I., Kulish, S., Shcherbyna, R., & Samura, T. (2019). Influence Of Amprolinsile And Bro-vitacoccid On The Protein Synthesizing Function Of The Liver And Enzyme Activity In Turkey Blood Serum During Eimeria Invasion. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 10(2), 723−729.

Gutyj, B., Grymak, Y., Drach, M., Bilyk, O., Matsjuk, O., Magrelo, N., Zmiya, M., & Katsaraba, O. (2017). The impact of en-dogenous intoxication on biochemical indicators of blood of pregnant cows. Regulatory Mechanisms in Biosystems, 8(3), 438–443. doi: 10.15421/021768.

Gutyj, B., Khariv, I., Binkevych, V., Binkevych, O., Levkivska, N., Levkivskyj, D., & Vavrysevich, Y. (2017). Research on acute and chronic toxity of the experimental drug Аmprolinsyl. Reg-ulatory Mechanisms in Biosystems, 8(1), 41–45. doi: 10.15421/021708.

Gutyj, B., Ostapiuk, A., Kachmar, N., Stadnytska O., Sobolev O., Binkevych V., Petryshak R., Petryshak O., Kulyaba, O., Naumyuk, A., Nedashkivsky, V., Nedashkivska, N., Magrelo, N., Golodyuk, I., Nazaruk, N., & Binkevych, O. (2019). The effect of cadmium loading on protein synthesis function and functional state of laying hens’ liver. Ukrainian Journal of Ecology, 9(3), 222–226. URL: https://www.ujecology.com/ ab-stract/the-effect-of-cadmium-loading-on-protein-synthesis-function-and-functional-state-of-laying-hens-liver-44516.html.

Kulyaba, O. V., Stybel, V. V., & Gutyj, B. V. (2016). The influence of clozaverm A and catozal on antioxidant status of cows organism for the experimental fasciolosis, sensitized atypical mycobacteria. Scientific Messenger LNUVMBT named after S. Z. Gzhytskyj, 18, 2(66), 96–99. doi: 10.15421/nvlvet6621.

Kulyaba, O., Stybel, V., & Gutyj, B. (2017). The influence of closaverm а and catosal on indicators of protein synthesizing functions of cows liver by experimental fasciolosis, sentsitized by atypical mycobacteria. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 19(73), 122–125. doi: 10.15421/nvlvet7325.

Kulyaba, O., Stybel, V., Gutyj, B., Turko, I., Peleno, R., Turko, Ya., Golovach, P., Vishchur, V., Prijma, O., Mazur, I., Dutka, V., Todoriuk, V., Golub, O. Dmytriv, O., & Oseredchuk, R. (2019). Effect of experimental fascioliasis on the protein synthesis function of cow liver. Ukrainian Journal of Ecology, 9(4), 612-615. URL: https://www.ujecology.com/articles/effect-of-experimental -fascioliasis-on-the-protein-synthesis-function-of-cow-liver.pdf.

Lavryshyn, Y. Y., & Gutyj, B. V. (2019). Protein synthesize func-tion of bulls liver at experimental chronic cadmium toxicity. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary sciences, 21(94), 92–96. doi: 10.32718/nvlvet9417.

Macs, L., Lauvers, H., Deckers, W., & Vanparijs, O. (1988). Flukicidal action of closantel against immature and mature Fasciola hepatica in experimentally infected rats and sheep. Res Vet Sci, 44(2), 229–232. doi: 10.1016/S0034-5288(18)30845-2.

Mas-Coma, S., Bargues, M. D., & Valero, M. A. (2005). Fascio-liasis and other plant-borne trematode zoonoses. International Journal for Parasitology, 35, 1255–1278. doi: 10.1016/j.ijpara.2005.07.010.

Matanović, K., Severin, K., Martinković, F., Šimpraga, M., Janicki, Z., & Barišić, J. (2007). Hematological and biochemical changes in organically farmed sheep naturally infected with Fasciola hepatica. Parasitol Res, 101(6), 1657‒1661. doi: 10.1007/s00436-007-0709-2.

Mitchell, G. (2002). Update on fasciolosis in cattle and sheep. In Practice, 24, 378–385. doi: 10.1136/inpract.24.7.378.

Moghaddam, A., Massoud, J., Mahmoodi, M., Mahvi, A. H., Peri-ago, M. V., Artigas, P., Fuentes, M. V., Bargues, M. D., & Mas-Coma, S. (2004). Human and animal fascioliasis in Ma-zandaran Province, northern Iran. Parasitol Res, 94(1), 61–69. doi: 10.1007/s00436-004-1169-6.

Mooney, L., Good, B., Hanrahan, J. P., Mulcahy, G., & de Waal, T. (2009). The comparative efficacy of four anthelmintics against a natural acquired Fasciola hepatica infection in hill sheep flock in the west of Ireland. Veterinary Parasitology, 164(2–4), 201–205. doi: 10.1016/j.vetpar.2009.05.017.

Said, W. S., Stybel, V. V., Gutyj, B. V., Pryima, O. B., & Mazur, I. Y. (2020). Protein-synthesizing function and functional state of the liver of dogs at experimental toxocariasis. Scientific Mes-senger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary sciences, 22(98), 132–137. doi: 10.32718/nvlvet9823.

Slivinska, L. G., Shcherbatyy, A. R., Lukashchuk, B. O., & Gutyj, B. V. (2020). The state of antioxidant protection system in cows under the influence of heavy metals. Regulatory Mechanisms in Biosystems, 11(2), 237–242. doi:10.15421/022035.

Vázquez, A. A., Lounnas, M., Sánchez, J., Alba, A., Milesi, A., & Hurtrez-Boussès, S. (2016). Genetic and infective diversity of the liver fluke Fasciola hepatica (Trematoda: Digenea) from Cuba. Journal of Helminthology, 90(6), 719725. doi: 10.1017/S0022149X15001029.

Vlizlo, V. V. (2012). Laboratorni metody doslidzhen u biologiyi, tvarynnycztvi ta veterynarnij medycyni: dovidnyk. Lviv: Spolom (in Ukrainian).
Published
2021-03-01
How to Cite
Frejuk, D., & Stybel, V. (2021). Protein-synthesizing function of the liver of cows at experimental fasciolosis. Ukrainian Journal of Veterinary and Agricultural Sciences, 4(1), 12-15. https://doi.org/https://doi.org/10.32718/ujvas4-1.03

Most read articles by the same author(s)