System of antioxidant protection of the body of piglets under the action of feed additive “Butaselmevit-plus”


  • T. V. Martyshuk Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Pekarska str., 50, Lviv, 79010, Ukraine https://orcid.org/0000-0002-8445-1794
  • B. V. Gutyj Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Pekarska str., 50, Lviv, 79010, Ukraine https://orcid.org/0000-0002-5971-8776
  • V. I. Khalak State Institution “Institute of Grain Crops of NAAS”, V. Vernadskoho str., 14, Dnipro, 49027, Ukraine https://orcid.org/0000-0002-4384-6394
Keywords: piglets, stress, antioxidant defense system, vitamins, selenium, methionine

Abstract

The aim of the study was to investigate the effect of the feed additive “Butaselmevit-plus” on the state of the antioxidant defense system of piglets during weaning. The experiments were conducted on the basis of the LTD “KOSHET” Mukachevo district of Zakarpattia region. Two groups of piglets were formed – control and experimental, in the amount of 10 individuals in each group, selected on the principle of analogs – age, breed and body weight. Animals were fed according to the norms for this age of pigs. On the 28th day of life, the piglets were weaned from the sow and regrouped from different nests for further maintenance during fattening and rearing with a change in the structure of the diet, which served as technological stress for the animal's body. Piglets of the experimental group, from 21 to 40 days of age, were additionally fed the feed additive “Butaselmevit-plus” at a dose of 100 mg/kg body weight per day. The material for the study was blood, which was taken in the morning before feeding the animals by puncture of the cranial vena cava for 20 days of life (the period before weaning), for 25 days of life (the period before weaning), for 30 days of life (2 days after weaning), for 35 days of life (7 days after weaning), 40 days of life (12 days after weaning). It is proved that feeding piglets feed additive “Butaselmevit-plus” enhanced the antioxidant status of piglets after weaning. It was found that under conditions of technological stress, the use of feed additive “Butaselmevit-plus” in the amount of 100 mg/kg body weight per day improves the antioxidant defense of the body, as evidenced by an increase in blood 35-day-old piglets superoxide dismutase activity by 26.7 % (P < 0.001), catalase – by 39.5 % (P < 0.001), glutathione peroxidase – by 51.7 % (P < 0.05), glutathione reductase – by 52.9 % (P < 0.05), the content of reduced glutathione – at 58.3 % (P < 0.001). These results confirm the effectiveness of the use of milk thistle, methionine, selenium, and vitamins A, E, and D3 in the feed additive “Butaselmevit-plus” to piglets in the period before and after weaning to activate the protective systems of their body.

References

Ahmed, S. T., Hoon, J., Mun, H., & Yang, C. (2014). Evaluation of Lactobacillus and Bacillus – based probiotics as alternatives to antibiotics in enteric microbial challenged weaned piglets. Afri-can Journal of Microbiology Research, 8(1), 96–104. doi: 10.5897/AJMR2013.6355.

Ariza-Nieto, C., Bandrick, M., Baidoo, S. K., Molitor, T. W., & Hathaway, M. R. (2011). Effect of dietary supplementation of oregano essential oils to sows on colostrum and milk composi-tion, growth pattern and immune status of suckling pigs. Journal of Animal Science, 89(4), 1079–1089. doi: 10.2527/jas.2010-3514.

Bulter, J., Sinkora, M., & Wertz, N. (2006). Development of the neonatal B and T cell repertoire in swine: implications for com-parative and veterinary immunology. Veterinary Research, 37(3), 417–441. doi: 10.1051/vetres:2006009.

Chen, X., Xu, J., Ren, E. Su, Y., & Zhu, W. (2018). Co-occurrence of early gut colonization in neonatal piglets with microbiota in the maternal and surrounding delivery environments. Anaerobe, 49, 30–40. doi: 10.1016/j.anaerobe.2017.12.002.

Czech, A., Smolczyk, A., & Ognik, K. (2018). Effect of dietary supplementation with Yarrowia lipolytica or Saccharomyces cerevisiae yeast and probiotic additives on haematological pa-rameters and the gut microbiota in piglets. Research in Veteri-nary Science, 119, 221–227. doi: 10.1016/j.rvsc.2018.06.007.

De Lange, C., Pluske, J. R., Gong, J., & Nyachoti, C. M. (2010). Strategic use of feed ingredients and feed additives to stimulate gut health and development in young pigs. Livestock Science, 134(1-3), 124–134. doi: 10.1016/j.livsci.2010.06.117.

Fedak, N., Chumachenko, S., Darmohray, L. M., Gutyj, B. V., & Perederiy, M. H. (2018). The effectiveness of the use of probiot-ics for wet maize grain preserving. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(89), 85–88. doi: 10.32718/nvlvet8916.

Frankic, T., Levart, A., & Salobir, J. (2010). The effect of vitamin E and plant extract mixture composed of carvacrol, cinnamalde-hyde and capsaicin on oxidative stress induced by high PUFA load in young pigs. Animal, 4(4), 572–578. doi: 10.1017/S1751731109991339.

Gutyj, B., Leskiv, K., Shcherbatyy, A., Pritsak, V., Fedorovych, V., Fedorovych, O., Rusyn, V., & Kolomiiets, I. (2017). The influ-ence of Metisevit on biochemical and morphological indicators of blood of piglets under nitrate loading. Regulatory Mechanisms in Biosystems, 8(3), 427–432. doi: 10.15421/021766.

Gutyj, B., Martyshchuk, T., Bushueva, I., Semeniv, B., Parchenko, V., Kaplaushenko, A., Magrelo, N., Hirkovyy, A., Musiy, L., & Murska, S. (2017). Morphological and biochemical indicators of blood of rats poisoned by carbon tetrachloride and subject to ac-tion of liposomal preparation. Regulatory Mechanisms in Bio-systems, 8(2), 304–309. doi: 10.15421/021748.

Gutyj, B., Stybel, V., Darmohray, L., Lavryshyn, Y., Turko, I., Hachak, Y., Shcherbatyy, A., Bushueva, I., Parchenko, V., Kaplaushenko, A., & Krushelnytska, O. (2017). Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load. Ukrainian Journal of Ecology, 7(4), 589–596.

Han, C., Dai,Y., Liu, B. Wang, L., Wang, J., & Zhang, J. (2019). Diversity analysis of intestinal microflora between healthy and diarrheal neonatal piglets from the same litter in different re-gions. Anaerobe, 55, 136–141. doi: 10.1016/j.anaerobe.2018.12.001.

Hedemann, M. S., & Jensen, B. B. (2004). Variations in enzyme activity in stomach and pancreatic tissue and digesta in piglets around weaning. Archives of Animal Nutrition, 58(1), 47–59. doi: 10.1080/00039420310001656677.

Heo, J. M., Opapeju, F. O., & Kim, J. C. (2012). Gastrointestinal health and function in weaned pigs: a review of feeding strate-gies to control post-weaning diarrhoea without using in-feed an-timicrobial compounds. Journal of Animal Physiology and Ani-mal Nutrition, 97(2), 207–237. doi: 10.1111/j.1439-0396.2012.01284.x.

Holman, D. B., & Chénierab, M. R. (2015). Antimicrobial use in swine production and its effect on the swine gut microbiota and antimicrobial resistance. Canadian Journal of Microbiology, 61(11), 785–798. doi: 10.1139/cjm-2015-0239.

Ivankiv, M., Kachmar, N., Mazurak, O., & Martyshuk, T. (2019). Hepatic protein synthesis and morphological parameters in blood of rats under oxidative stress and action of feed additive “Bu-taselmevit-plus”. Ukrainian Journal of Ecology, 9(4), 628–633. URL: https://www.ujecology.com/abstract/hepatic-protein-synthesis-and-morphological-parameters-in-blood-of-rats-under-oxidative-stress-and-action-of-feed-additi-44975.html.

Jacela, J. Y., DeRouchey, J. M., & Tokach, M. D. (2010). Feed additives for swine: Fact sheets – prebiotics and probiotics, and phytogenics. Kansas Agricultural Experiment Station Research Reports, 18(3), 132–136. doi: 10.4148/2378-5977.7067.

Jayaraman, B., & Nyachoti, C. M. (2017). Husbandry practices and gut health outcomes in weaned piglets: A review. Animal Nutrition, 3(3), 205–211. doi: 10.1016/j.aninu.2017.06.002.

Khalak, V., & Gutyj, B. (2020). Physicochemical properties and chemical composition of muscle tissue of young pigs of large white breed and their correlation with some serum enzymes. Ukrainian Journal of Veterinary and Agricultural Sciences, 3(3), 34–38. doi: 10.32718/ujvas3-3.07.

Khalak, V., Gutyj, B., Bordun, O., Ilchenko, M., & Horchanok, A. (2020). Effect of blood serum enzymes on meat qualities of piglet productivity. Ukrainian Journal of Ecology, 10(1), 158–161. doi: 10.15421/2020_25.

Khalak, V., Gutyj, B., Bordun, О., Horchanok, A., Ilchenko, M., Smyslov, S., Kuzmenko, O., & Lytvyshchenko, L. (2020). De-velopment and reproductive qualities of sows of different breeds: innovative and traditional methods of assessment. Ukrainian Journal of Ecology, 10(2), 356–360. doi: 10.15421/2020_109.

Khariv, M., Gutyj, B., Butsyak, V., & Khariv, I. (2016). Hematolog-ical indices of rat organisms under conditions of oxidative stress and liposomal preparation action. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(1), 276–289. doi: 10.15421/201615.

Khariv, M., Gutyj, B., Ohorodnyk, N., Vishchur, O., Khariv, I., Solovodzinska, I., Mudrak, D., Grymak, C., & Bodnar, P. (2017). Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug. Ukrainian Journal of Ecology, 7(4), 536–541. doi: 10.15421/2017_157.

Kiczorowska, B., Samolinska, W., & Al-Yasiry, A. R. M. (2017). The natural feed additives as immunostimulants in monogastric animal nutrition – a review. Annals of Animal Science, 17(3), 605–625. doi: 10.1515/aoas-2016-0076.

Kramarenko, S. S., Lugovoy, S. I., Kharzinova, V. R., Lykhach, V., Kramarenko, A. S., & Lykhach, A. V. (2018). Genetic diversity of Ukrainian local pig breeds based on microsatellite markers. Regulatory Mechanisms in Biosystems, 9(2), 177–182. doi: 10.15421/021826.

Kramarenko, S., Lugovoy, S., Lykhach, A., Kramarenko, A., & Lykhach, V. (2018). A comparative study of the reproductive traits and clustering analysis among different pig breeds. Scien-tific Messenger of LNU of Veterinary Medicine and Biotechnol-ogies. Series: Agricultural Sciences, 20(84), 21–26. doi: 10.15421/nvlvet8404.

Kramarenko, S., Lugovoy, S., Lykhach, A., Kramarenko, A., Lykhach, V., & Slobodianyk, A. (2019). Effect of genetic and non-genetic factors on the reproduction traits in Ukrainian Meat sows. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Agricultural Sciences, 21(90), 3–8. doi: 10.32718/nvlvet-a9001.

Lavryshyn, Y. Y., Varkholyak, I. S., Martyschuk, T. V., Guta, Z. А., Ivankiv, L. B., Paladischuk, О. R., Murska, S. D., Gutyj, B. V., & Gufriy, D. F. (2016). The biological significance of the antioxidant defense system of animals body. Scientific Messen-ger LNUVMBT named after S. Z. Gzhytskyj, 18(2(66), 100–111. doi: 10.15421/nvlvet6622.

Leskiv, Kh. Ya., Gufriy, D. F., Gutyj, B. V., Khalak, V. I., Hariv, I. I., Martyshuk, T. V., & Guta, Z. A. (2021). The effect of methi-phene, methionine, and phenarone on the humoral part of the immune System of piglets in experimental chronic nitrate-nitrite toxicosis. Colloquium-journal, 7(94), 12‒15. doi: 10.24412/2520-6990-2021-794-12-15.

Lukashchuk, B. O., Slivinska, L. G., Shcherbatyy, A. R., Zinko, H. O., & Gutyj, B. V. (2020). Influence of modern treatment regimens on serum biochemical parameters in piglets with gastroenteritis. Regulatory Mechanisms in Biosystems, 11(1), 67–73. doi: 10.15421/022009.

Lykhach, A. V., Lykhach, V. Y., Shpetny, M. B., Mykhalko, O. H., & Zhyzhka, S. V. (2020). Influence of toys on behavioural patterns of pigs and their association with the concentration of serotonin in blood plasma. Regulatory Mechanisms in Biosystems, 11(1), 146–150. doi: 10.15421/022022.

Martyshuk, T. V. (2016). Vplyv oksydatsiinoho stresu na systemu antyoksydantnoho zakhystu orhanizmu shchuriv. Visnyk Dnipropetrovskoho universytetu. Biolohiia, medytsyna. Dnipro, 7(1), 8–12 (in Ukrainian).

Martyshuk, T. V., & Gutyi, B. V. (2019). Influence of feed additive «Butaselmevit Plus» on the indicators of rats blood under the conditions of their poisoning with Tetrachloromethane. Theoret-ical and Applied Veterinary Medicine, 7(2), 79‒83. doi: 10.32819/2019.71014.

Martyshuk, T. V., & Hutyi, B. V. (2019). Morfolohichni pokaznyky krovi shchuriv za umov oksydatsiinoho stresu ta za dii kormovoi dobavky “Butaselmevit-plius”. Naukovo-tekhnichnyi biuleten Derzhavnoho naukovo-doslidnoho kontrolnoho instytutu vet-erynarnykh preparativ ta kormovykh dobavok i Instytutu biolohii tvaryn. Lviv, 20(2), 94‒104 (in Ukrainian).

Martyshuk, T. V., & Hutyi, B. V. (2021). Imunofiziolohichnyi stan ta antyoksydantnyi potentsial orhanizmu porosiat za umov oksydatsiinoho stresu ta dii koryhuiuchykh chynnykiv: mon-ohrafiia. Lviv: SPOLOM (in Ukrainian).

Martyshuk, T. V., Gutyj, B. V., & Vishchur, O. I. (2016). Level of lipid peroxidation products in the blood of rats under the influ-ence of oxidative stress and under the action of liposomal prepa-ration of ‘‘Butaselmevit’’. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(2), 22–27. doi: 10.15421/201631.

Martyshuk, T. V., Gutyj, B. V., Leskiv, Kh. Ya., Semaniuk, N. V., & Shnaider, V. L. (2021). The effect of the liposomal drug “bu-taselmevit” on the enzymatic and non-enzymatic part of the glu-tatoin system of the blood of rats under oxidative stress. Collo-quium-journal, 12(99), 16‒19.

Martyshuk, T. V., Gutyj, B. V., Zhelavskyi, M. M., Midyk. S. V., Fedorchenko, A. M., Todoriuk, V. B., Nahirniak, T. B., Kisera, Ya. V., Sus, H. V., Chemerys, V. A., Levkivska, N. D., & Ig-litskej, I. I. (2020). Effect of Butaselmevit-Plus on the immune system of piglets during and after weaning. Ukrainian Journal of Ecology, 10(2), 347‒352. doi: 10.15421/2020_106.

Martyshuk, T. V., Hutyi, B. V., Khalak, V. I., Stadnytska, O. I., & Todoriuk, V. B. (2019). Stan imunnoi systemy porosiat za dii kormovoi dobavky “Butaselmevit-plius”. Visnyk Poltavskoi derzhavnoi ahrarnoi akademii, 4, 116‒125. doi: 10.31210/visnyk2019.04.14 (in Ukrainian).

Martyshuk, T., Gutyj, B., Vishchur, O., & Todoriuk, V. (2019). Biochemical indices of piglets blood under the action of feed ad-ditive “Butaselmevit-plus”. Ukrainian Journal of Veterinary and Agricultural Sciences, 2(2), 27–30. doi: 10.32718/ujvas2-2.06.

Masiuk, D. M., Sosnitsky, O. I., Nedzvetsky, V. S., Kokarev, A. V., & Koliada, S. G. (2017). Epidemiology, etiology and gene analysis of spike S protein of porcine epidemic diarrhea virus infection in Ukraine during 2016–2017. Regulatory Mechanisms in Biosystems, 8(4), 602–610. doi: 10.15421/021792.

Masiuk, D. N., Nedzvetsky, V. S., Sosnitskiy, A. I., Kokarev, A. V., & Koliada, S. G. (2018). The characteristics, emergent properties and manner of spread in Ukraine of the Porcine Epidemic Diarrhea Virus. Regulatory Mechanisms in Biosystems, 9(3), 401–408. doi: 10.15421/021860.

Nazaruk, N. V., Gutyj, B. V., Gufrij, D. F., Leskiv, Kh. Ya., Ivashkiv, R. M., & Martyshuk, T. V. (2021). The effect of methyphen and vitamix se on the level of products of bull lipid peroxide oxidation under nitrate-cadmium load. Colloquium-journal, 7(94), 16–18. doi: 10.24412/2520-6990-2021-794-16-18.

Sachuk, R., Stravskyy, Y., Gutyj, B., Velesyk, T., Katsaraba, O., & Zhyhaliuk, S. (2021). Study of acute toxicity of the drug “Kolidev 8M” with a single intragastric injection in laboratory animals. ScienceRise: Biological Science, 2(27), 44–48. doi: 10.15587/2519-8025.2021.235952.

Slobodian, S. О., Gutyj, B. V., Darmohray, L. M., & Povoznikov, M. G. (2021). Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors. Regulatory Mechanisms in Biosystems, 12(2), 315–320. doi: 10.15421/022142.

Snegin, E. A., Kramarenko, A. S., Artemchuk, O. Y., & Kramarenko, S. S. (2021). Intra- and interbreed genetic heterogeneity and divergence in four commercial pig breeds based on microsatellite markers. Regulatory Mechanisms in Biosystems, 12(1), 128–135. doi: 10.15421/022120.

Sobolev, А., Gutyj, B., Grynevych, N., Bilkevych, V., & Mashkin, Y. (2017). Enrichment of meat products with selenium by its introduction to mixed feed compounds for birds. Regulatory Mechanisms in Biosystems, 8(3), 417–422. doi: 10.15421/021764.

Stoyanovskyy, V., Usenko, S., Shostya, A., Kuzmenko, L., Slynko, V., & Tenditnyk, V. (2020). Hormonal regulation of prooxidant-antioxidant homeostasis in gilts. Ukrainian Journal of Veterinary and Agricultural Sciences, 3(3), 39–43. doi: 10.32718/ujvas3-3.08.

Stybel, V. V., Gutyj, B. V., Said, W. S., Kubiak, K., Jankowski M., Maksymovych, I. A., Guta, Z. A., Martyshuk, T. V., & Karpov-skiy, V. I. (2021). The effect of fenbensyl and fenbendazole on the antioxidant status of dogs during experimental invasion with the pathogen toxocariasis. Ukrainian Journal of Veterinary Sci-ences, 12(2). URL: http://journals.nubip.edu.ua/index.php/ Veterenarna/article/view/15141.

Varkholiak, I. S., Gutyj, B. V., Leskiv, Kh. Ya., Kushnir, V. I., Hariv, I. I., Martyshuk, T. V., & Guta, Z. A. (2021). The effect of bendamine on antioxidant protection of rats' myocardium in doxorubicin intoxication. Colloquium-journal, 7(94), 18–21. doi: 10.24412/2520-6990-2021-794-18-21.

Vasylyev, D., Priimenko, B., Aleksandrova, K., Mykhalchenko, Y., Gutyj, B., Mazur, I., Magrelo, N., Sus, H., Dashkovskyy, O., Vus, U., & Kamratska, O. (2021). Investigation of the acute tox-icity of new xanthine xenobiotics with noticeable antioxidant ac-tivity. Ukrainian Journal of Ecology, 11(1), 315–318. doi: 10.15421/2021_47.
Published
2021-06-21
How to Cite
Martyshuk, T., Gutyj, B., & Khalak, V. (2021). System of antioxidant protection of the body of piglets under the action of feed additive “Butaselmevit-plus”. Ukrainian Journal of Veterinary and Agricultural Sciences, 4(2), 38-43. https://doi.org/https://doi.org/10.32718/ujvas4-2.07

Most read articles by the same author(s)