The comparative evaluation of complex drugs based on fipronil for ectoparasitosis of dogs and cats


  • O. L. Tishyn State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Donetska Str., 11, Lviv, 79019, Ukraine https://orcid.org/0000-0001-8102-8424
  • I. D. Yuskiv Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Pekarska Str., 50, Lviv, 79010, Ukraine https://orcid.org/0000-0002-6029-3488
  • L. L. Yuskiv Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Pekarska Str., 50, Lviv, 79010, Ukraine https://orcid.org/0000-0003-4642-7520
  • Zn. M. Perih State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Donetska Str., 11, Lviv, 79019, Ukraine https://orcid.org/0000-0002-6317-4249
  • O. M. Bogach State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Donetska Str., 11, Lviv, 79019, Ukraine https://orcid.org/0000-0001-5487-7033
Keywords: parasitology, dogs, cats, siphonapterosis, ixodidosis, Otodectesis, the effectiveness of the drug

Abstract

The article presents data on the effectiveness of new domestic antiparasitic drug “Insectostop for dogs and cats” in comparison to the reference drugs “Burdi Fipro for dogs” and “Burdi Fipro for cats” that is used for therapeutic and prophylactic purposes against ectoparasitosis of dogs and cats of different breeds. According to the results of the research, the parasitism of fleas of Ctenocephalus canis, C. felis and Pulex irritans species and parasitiform mites of the Ixodes ricinus and Dermacentor reticulatus species and sarcoptiform mites of the species Otodectes cynotis were detected on experimental animals. Clinically, this was inspected by redness, inflammation of the skin, itching reflex, the emergence of papules on the skin, scales. Based on the results, it was found that 8 hours after usage of the experimental drug “Insectostop for dogs and cats” its effectiveness in syphonapterosis of dogs was 87.9 %, and after usage of the reference drug “Bourdie Fipro for dogs” – 86.9 %, and in experiments on cats, the effectiveness of the experimental drug for siphonapterosis in cats was 92.4 %, and the reference drug “Bourdie Fipro for cats” – 90.3 %. Starting from the first and third days after usage of drugs on the fur of dogs and cats of the experimental and control groups, parasitological studies did not reveal fleas of the species Ctenocephalus canis, C. felis and Pulex irritans. So starting from the first day, the experimental and reference drugs showed 100 % effectiveness during the siphonapterosis in dogs and cats. During the ixodidosis of dogs, it was found that at the 8-th hour after usage of the experimental drug “Insectostop for dogs and cats” its effectiveness was 82.9 %, and after usage of the reference drug “Bourdie Fipro for dogs” – 81.3 %. At the 24-th hour after usage of the experimental drug, its effectiveness was 97.6 %, and after usage of the reference drug – 95.9 %. At the 72nd hour of the experiment, no adult representatives of the species Ixodes ricinus and Dermacentor reticulatus were found on the fur of dogs of these groups. Thus, at 72 hours after usage of the experimental and reference drug, they showed 100 % efficiency on adult Ixodes mites. During the Otodectesis of dogs it was found that on the 7th day, effectiveness of the experimental drug was 75.6 %, on the 14th day – 98.1 % and after usage of the reference drug “Bourdi Fipro for dogs” its effectiveness on the 7th day was 73.5 %, and on the 14th – 98.3 %. At 21-st days after treatment of animals with drugs as a result of clinical examination and parasitological examination of dogs mites of the species Otodectes cynotis were not detected. In experiments on cats, on the 7th day after usage of experimental drug, its effectiveness during Otodectesis of cats was 76.9 %, and after usage of reference drug – 77.1 %. As a result of clinical examination and parasitological study of the experimental and control groups of animals on the 14th day after treatment of the auricles of cats with mites of the species Otodectes cynotis was not detected. Thus, usage of both drugs promotes the release of Otodectesis in dogs from parasites on the 21st day of the experiment, and cats – on the 14th day. Tests have shown that the experimental drug “Insectostop for dogs and cats” (100 ml of the drug contains the active substance: fipronil – 10 g) does not cause skin irritation, dermatitis, seborrhea, allergic and other side effects, that the drug is well tolerated by dogs and cats and doesn’t give any side effects and changes of clinical condition of animals.

References

Beugnet, F., & Franc, M. (2010). Results of a European multicentric field efficacy study of fipronil-(S) methoprene combination1 on flea infestation of dogs and cats during 2009 summer. Parasite, 17(4), 337–342. doi: 10.1051/parasite/2010174337.

Craig, M. (2016). Surface mites in dogs, cats and rabbits. Compan-ion animal, 21(12), 678–684. doi: 10.12968/coan.2016.21.12.678.

ESCCAP (2018). Control of Ectoparasites in Dogs and Cats. Guide-line 03 Sixth Edition, 1–34. URL: https://www.esccap.org/uploads/ docs/mjy50wev_0720_ESCCAP_Guideline_GL3_v9_1p.pdf.

European Medicines Agency, Committee for Medicinal Products for Veterinary Use, EMEA/CVMP/EWP/005/2000-Rev.2: Guide-line for the testing and evaluation of the efficacy of antiparasitic substances for the treatment and prevention of mite and flea in-festation in dogs and cats, 12 November 2007. London: Europe-an Medicines Agency; 2007. URL: https://www.ema.europa.eu/en/documents/scientific-guideline/guideline-testing-evaluation-efficacy-antiparasitic-substances-treatment-prevention-tick-flea_en.pdf.

European Medicines Agency, Committee for Medicinal Products for Veterinary Use, EMEA/CVMP/EWP/005/2000-Rev.3: Guide-line for the testing and evaluation of the efficacy of antiparasitic substances for the treatment and prevention of tick and flea in-festation in dogs and cats. URL: http://www.ema.europa.eu/docs/en_GB/document_library/Scientific_guideline/2016/07/WC500210927.pdf.

Hnilica, K., & Patterson, A. (2016). Small Animal Dermatology 4th Edition. A Color Atlas and Therapeutic Guide. URL: https://www.elsevier.com/books/small-animal-dermatology/ hnilica/978-0-323-37651-8.

International Cooperation on Harmonization of Technical Require-ments for Registration of Veterinary Medicinal Products. VICH GL9, Good Clinical Practice. London: European Medicines Agency; 2000. URL: https://www.ema.europa.eu/ en/vich-gl9-good-clinical-practices.

Kramer, F., & Mencke, N. (2001). Flea Biology and Control. Springer – Berlin – New York.

Linardi, P. M., & Santos, L. (2012). Ctenocephalides felis felis vs. Ctenocephalides canis (Siphonaptera: Pulicidae): some issues in correctly identify these species. Rev. Bras. Parasitol. Vet., 21(4), 345–354. doi: 10.1590/s1984-29612012000400002.

Marchiondo, A. A., Holdsworth, P. A., Fourie, L. J., Rugg, D., Hellmann, K., Snyder, D. E., & Dryden, M. W. (2013). World Association for the Advancement of Veterinary Parasitology. World Association for the Advancement of Veterinary Parasit-ology (W.A.A.V.P.) second edition: guidelines for evaluating the efficacy of parasiticides for the treatment, prevention and control of flea and tick infestations on dogs and cats. Vet. Parasitol., 194(1), 84–97. doi: 10.1016/j.vetpar.2013.02.003.

Rusr, M. K. (2017). The Biology and Ecology of Cat Fleas and Advancements in Their Pest Management: A Review. Insects, 118, 1–51. doi: 10.3390/insects8040118.

Statistical methodology, clinical trial design, veterinary medicinal product (VMP). CVMP/EWP/81976/2010. European Medicines Agency. 16 January 2012. URL: https://www.ema.europa.eu/en/documents/scientific-guideline/final-guideline-demonstration-efficacy-veterinary-medicinal-products-containing-antimicrobial_en.pdf.

Taylor, M. A., Coop, R. L., & Wall, R. L. (2016). Veterinary para-sitology. – Wiley Blackwell. This edition first published.

Yuskiv, I. D. (1998). Akarologichni doslidchenna tvarin ta akara-cidi. Navch.-prakt. pocibnik. Lviv, Kamenar. URL: https://www.researchgate.net/profile/Igor-Yuskiv-2/publication/ 327890520_Akarologicni_doslidzenna_tvarin_ta_akaricidi_Navc-_prakt_posibnik/links/5bab7f98299bf13e604cdda3/Akarologicni-doslidzenna-tvarin-ta-akaricidi-Navc-prakt-posibnik.pdf (in Ukrainian).
Published
2021-08-17
How to Cite
Tishyn, O., Yuskiv, I., Yuskiv, L., Perih, Z., & Bogach, O. (2021). The comparative evaluation of complex drugs based on fipronil for ectoparasitosis of dogs and cats. Ukrainian Journal of Veterinary and Agricultural Sciences, 4(2), 52-57. https://doi.org/https://doi.org/10.32718/ujvas4-2.09